機能Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
組織特異性CNS stem cells.
配列類似性Belongs to the intermediate filament family.
発生段階Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
翻訳後修飾Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
ICC/IF image of ab82375 stained SKNSH cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82375, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-Nestin antibody (ab82375) 使用論文
has not yet been referenced specifically in any publications.