製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Nestin
  • アプリケーション
    適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S1409 of human Nestin.

  • ポジティブ・コントロール
    • HepG2 cell line lysates. IF/ICC: SKNSH cell line

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50 - 1/100. Predicted molecular weight: 177 kDa.
ELISA 1/1000.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
  • 組織特異性
    CNS stem cells.
  • 配列類似性
    Belongs to the intermediate filament family.
  • 発生段階
    Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
  • 翻訳後修飾
    Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ESTM 46 antibody
    • FLJ 21841 antibody
    • FLJ21841 antibody
    • Intermediate filament protein antibody
    • Nbla00170 antibody
    • nes antibody
    • NEST_HUMAN antibody
    • Nestin antibody
    see all

画像

  • Anti-Nestin antibody (ab82375) at 1/50 dilution + HepG2 cell line lysates at 35 µg

    Predicted band size : 177 kDa
    Observed band size : 177 kDa
    Additional bands at : 60 and 65 kDa,95 and 120 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab82375 stained SKNSH cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82375, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Jiao Y  et al. Resveratrol Inhibits the Invasion of Glioblastoma-Initiating Cells via Down-Regulation of the PI3K/Akt/NF-?B Signaling Pathway. Nutrients 7:4383-402 (2015). ICC/IF ; Human . Read more (PubMed: 26043036) »

See 1 Publication for this product

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