Anti-Nestin 抗体 [7A3] (ab81462)
Key features and details
- Rat monoclonal [7A3] to Nestin
- Suitable for: ICC/IF
- Reacts with: Mouse
- Isotype: IgG2b
製品の概要
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製品名
Anti-Nestin antibody [7A3]
Nestin 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [7A3] to Nestin -
由来種
Rat -
アプリケーション
適用あり: ICC/IFmore details
適用なし: WB -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Neural progenitor cells from mouse fetal brain.
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特記事項
ab81462 is very useful for immunostaining of mouse embryonic brain. It has very low background in immunostaining using mouse tissues and is also useful for doubleb staining with mouse and rabbit antibodies.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6
Constituents: 50% Glycerol, PBS -
Concentration information loading...
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精製度
Ion Exchange Chromatography -
特記事項(精製)
The antibody was produced from the hybridoma cultured in serum free medium and purified under mild conditions by propriety chromatography processes. It is filter sterilized. -
一次抗体 備考
ab81462 is very useful for immunostaining of mouse embryonic brain. It has very low background in immunostaining using mouse tissues and is also useful for doubleb staining with mouse and rabbit antibodies. -
ポリ/モノ
モノクローナル -
クローン名
7A3 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81462の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
Use at an assay dependent concentration.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. -
組織特異性
CNS stem cells. -
配列類似性
Belongs to the intermediate filament family. -
発生段階
Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments. -
翻訳後修飾
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. - Information by UniProt
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参照データベース
- Entrez Gene: 18008 Mouse
- SwissProt: Q6P5H2 Mouse
- Unigene: 331129 Mouse
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別名
- ESTM 46 antibody
- FLJ 21841 antibody
- FLJ21841 antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab81462 は 14 報の論文で使用されています。
- Locatelli M et al. Empagliflozin protects glomerular endothelial cell architecture in experimental diabetes through the VEGF-A/caveolin-1/PV-1 signaling pathway. J Pathol 256:468-479 (2022). PubMed: 35000230
- Luo J et al. Repetitive Transcranial Magnetic Stimulation Improves Neurological Function and Promotes the Anti-inflammatory Polarization of Microglia in Ischemic Rats. Front Cell Neurosci 16:878345 (2022). PubMed: 35496902
- Buelli S et al. Shiga Toxin 2 Triggers C3a-Dependent Glomerular and Tubular Injury through Mitochondrial Dysfunction in Hemolytic Uremic Syndrome. Cells 11:N/A (2022). PubMed: 35681450
- Locatelli M et al. Sirtuin 3 Deficiency Aggravates Kidney Disease in Response to High-Fat Diet through Lipotoxicity-Induced Mitochondrial Damage. Int J Mol Sci 23:N/A (2022). PubMed: 35955472
- Wysmolek PM et al. A minimal-complexity light-sheet microscope maps network activity in 3D neuronal systems. Sci Rep 12:20420 (2022). PubMed: 36443413