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A recombinant 150 amino acid fragment from human Nestin conjugated to glutathione S transferase.
Monoclonal antibody 10C2 was derived by fusion of P3x63Ag8.653 myeloma cells with spleen cells from a BALB/c mouse immunized with a 150-amino-acid fragment from the cloned human nestin, conjugated to glutathione S-transferase.
Our Abpromise guarantee covers the use of ab22035 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/200. PubMed: 19147502
|IHC-Fr||1/100 - 1/200.|
|IHC-P||Use at an assay dependent concentration.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 176 kDa.
1/1000 - 1/5000 using chemiluminescent detection. Predicted molecular weight: 176 kDa.
ab22035 staining human brain tissue cells by ICC/IF. Cells were PFA fixed and permeabilized in Triton X-100 prior to blocking in 1% BSA for 30 minutes at 21°C. The primary antibody was diluted 1/100 and incubated with the samples for 1 hour at 21°C. A FITC conjugated goat anti-mouse IgG antibody, diluted 1/250, was used as the secondary.
ICC/IF of U251 cells staining intracellular Nestin filaments with ab22035