製品の概要

  • 製品名
    Anti-Nesprin3 antibody [Nsp3]
    Nesprin3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [Nsp3] to Nesprin3
  • アプリケーション
    適用あり: WB, IHC-P, IHC-Fr, ICC/IFmore details
  • 種交差性
    交差種: Mouse
    非交差種: Human, Zebrafish
  • 免疫原

    Fusion protein encoding the seventh spectrin repeat of Nesprin3 and a proprietary protein.

  • ポジティブ・コントロール
    • Mouse embryonic fibroblasts.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    Nsp3
  • ミエローマ
    Sp2/0
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab123031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 122 kDa. FITC-conjugated secondary antibodies or avidin-biotinylated horseradish peroxidase complex (ABC) may be used as detection reagents.
IHC-P 1/100 - 1/1000.
IHC-Fr 1/100 - 1/1000. fixed with methanol
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system.
  • 配列類似性
    Belongs to the nesprin family.
    Contains 1 KASH domain.
    Contains 2 spectrin repeats.
  • ドメイン
    The KASH domain is involved in the binding to SUN1 and SUN2 through recognition of their SUN domains.
  • 細胞内局在
    Nucleus outer membrane. Located at the rough reticulum endoplasmic (RER) in some cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C14orf49 antibody
    • Chromosome 14 open reading frame 49 antibody
    • FLJ25605 antibody
    • MGC75267 antibody
    • Nesp3 antibody
    • Nesprin 3 antibody
    • Nesprin-3 antibody
    • NET53 antibody
    • Nuclear Envelope Spectrin Repeat Protein 3 antibody
    • Spectrin Repeat Containing, Nuclear Envelope Family Member 3 antibody
    • SYNE3 antibody
    • SYNE3_HUMAN antibody
    see all

Anti-Nesprin3 antibody [Nsp3] 画像

  • ab123031 staining Nesprin3 in cultured mouse embryonic fibroblast by Immunofluorescence (green). The nuclear DNA is counterstained with DAPI (blue).

Anti-Nesprin3 antibody [Nsp3] (ab123031) 使用論文

This product has been referenced in:
  • Alam SG  et al. The mammalian LINC complex regulates genome transcriptional responses to substrate rigidity. Sci Rep 6:38063 (2016). ICC/IF . Read more (PubMed: 27905489) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab123031.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録