The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Predicted molecular weight: 122 kDa. FITC-conjugated secondary antibodies or avidin-biotinylated horseradish peroxidase complex (ABC) may be used as detection reagents.
1/100 - 1/1000.
1/100 - 1/1000. fixed with methanol
Use at an assay dependent concentration.
Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system.
Belongs to the nesprin family. Contains 1 KASH domain. Contains 2 spectrin repeats.
The KASH domain is involved in the binding to SUN1 and SUN2 through recognition of their SUN domains.
Nucleus outer membrane. Located at the rough reticulum endoplasmic (RER) in some cells.