製品の概要

  • 製品名
    Anti-Neprilysin-2 antibody
    Neprilysin-2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Neprilysin-2
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to Human Neprilysin-2 aa 371-420 (internal sequence).
    Sequence:

    EVVVYGIPYLQNLENIIDTYSARTIQNYLVWRLVLDRIGSLSQRFKDTRV


    Database link: NP_258428

  • ポジティブ・コントロール
    • Fetal liver lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 89 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

ターゲット情報

  • 機能
    Metalloprotease involved in sperm function, possibly by modulating the processes of fertilization and early embryonic development. Degrades a broad variety of small peptides with a preference for peptides shorter than 3 kDa containing neutral bulky aliphatic or aromatic amino acid residues. Shares the same substrate specificity with MME and cleaves peptides at the same amide bond.
  • 組織特異性
    Predominantly expressed in testis. Weakly expressed in brain, kidney and heart.
  • 配列類似性
    Belongs to the peptidase M13 family.
  • 翻訳後修飾
    N-glycosylated.
  • 細胞内局在
    Membrane. Secreted. A secreted form produced by proteollytic cleavage also exists.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Mel transforming oncogene like 1 antibody
    • Membrane metallo endopeptidase like 1 antibody
    • Membrane metallo endopeptidase like 2 antibody
    • Membrane metallo-endopeptidase-like 1 antibody
    • Membrane metallo-endopeptidase-like 2 antibody
    • MMEL1 antibody
    • MMEL1_HUMAN antibody
    • NEP2 antibody
    • NEP2(m) antibody
    • NEP2(s) antibody
    • NEPII antibody
    • Neprilysin 2 antibody
    • Neprilysin II antibody
    • Neprilysin Like 1 antibody
    • Neprilysin-2 antibody
    • Neprilysin-2 secreted antibody
    • Neprilysin-like Protease beta antibody
    • Neprilysin-like Protease gamma antibody
    • NL1 antibody
    • NL2 antibody
    • SEP antibody
    • soluble form antibody
    • Soluble secreted endopeptidase antibody
    see all

画像

  • Anti-Neprilysin-2 antibody (ab81688) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 89 kDa
    Observed band size : 89 kDa
    12% gel

プロトコール

参考文献

ab81688 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Rat Tissue lysate - whole (Testis and Brain)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
100 µg
Specification
Testis and Brain
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Renato Watanabe de Oliveira

Verified customer

投稿 Oct 29 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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