製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Nephrin
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A 14 amino acid peptide from near the carboxy terminus of human Nephrin.

  • ポジティブ・コントロール
    • WB: HEK293 and Mouse kidney lysate. IHC: Mouse kidney tissue.
  • 特記事項
    Although there are publications stating this antibody works with Rat species in WB (PMID 19052104). The antibody however fail to stain the Rat kidney sections hence we have removed the species(from customer feedback). Further feedback using this antibody with Rat tissues would be very welcome

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 138 kDa).

ターゲット情報

  • 機能
    Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.
  • 組織特異性
    Specifically expressed in podocytes of kidney glomeruli.
  • 関連疾患
    Defects in NPHS1 are the cause of nephrotic syndrome type 1 (NPHS1) [MIM:256300]; also known as Finnish congenital nephrosis (CNF). A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure.
  • 配列類似性
    Belongs to the immunoglobulin superfamily.
    Contains 1 fibronectin type-III domain.
    Contains 8 Ig-like C2-type (immunoglobulin-like) domains.
  • 発生段階
    In 23-week-old embryo found in epithelial podocytes of the periphery of mature and developing glomeruli.
  • 翻訳後修飾
    Phosphorylated on tyrosine residues.
  • 細胞内局在
    Cell membrane. Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CNF antibody
    • Nephrin antibody
    • Nephrosis 1 congenital Finnish type antibody
    • Nephrosis 1, congenital, Finnish type (nephrin) antibody
    • NPHN antibody
    • NPHN_HUMAN antibody
    • NPHS 1 antibody
    • Nphs1 antibody
    • Renal glomerulus specific cell adhesion receptor antibody
    • Renal glomerulus-specific cell adhesion receptor antibody
    see all

画像

  • All lanes : Anti-Nephrin antibody (ab58968) at 1 µg/ml

    Lane 1 : mouse kidney tissue lysate
    Lane 2 : mouse kidney tissue lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 138 kDa
    Observed band size: 100 kDa (why is the actual band size different from the predicted?)



    The difference in MW size could be due to modification to the protein or just aberrant migration.
  • ab58968, at a concentration of 1 µg/ml, staining Nephrin in mouse kidney tissue by Immunohistochemistry.
  • All lanes : Anti-Nephrin antibody (ab58968)

    Lane 1 : HEK293 cell lysate
    Lane 2 : Mouse kidney lysate

    Predicted band size: 138 kDa

  • Anti-Nephrin antibody (ab58968) at 0.5 µg/ml + Mouse kidney tissue lysate at 56 µg with Milk at 5 %

    Secondary
    HRP conjugated Goat anti-rabbit IgG monoclonal at 1/1000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 138 kDa
    Observed band size: 85 kDa (why is the actual band size different from the predicted?)


    Exposure time: 30 seconds

    See Abreview

参考文献

This product has been referenced in:
  • Zhang L  et al. PGC-1a ameliorates kidney fibrosis in mice with diabetic kidney disease through an antioxidative mechanism. Mol Med Rep 17:4490-4498 (2018). Read more (PubMed: 29344670) »
  • van de Lest NA  et al. Nephrin Loss Can Be Used to Predict Remission and Long-term Renal Outcome in Patients With Minimal Change Disease. Kidney Int Rep 3:168-177 (2018). Read more (PubMed: 29340328) »

See all 32 Publications for this product

レビューと Q&A

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
56 µg
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycin)
Sample
Mouse Tissue lysate - whole (Kidney)
Specification
Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (kidney)
Loading amount
10 µg
Specification
kidney
Gel Running Conditions
Non-reduced Denaturing (7.5%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Hongjie Wang

Verified customer

投稿 Mar 24 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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