A 14 amino acid peptide from near the carboxy terminus of human Nephrin.
WB: HEK293 and Mouse kidney lysate.
IHC: Mouse kidney tissue.
Although there are publications stating this antibody works with Rat species in WB (PMID 19052104). The antibody however fail to stain the Rat kidney sections hence we have removed the species(from customer feedback). Further feedback using this antibody with Rat tissues would be very welcome
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 138 kDa).
Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.
Specifically expressed in podocytes of kidney glomeruli.
Defects in NPHS1 are the cause of nephrotic syndrome type 1 (NPHS1) [MIM:256300]; also known as Finnish congenital nephrosis (CNF). A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure.
Belongs to the immunoglobulin superfamily. Contains 1 fibronectin type-III domain. Contains 8 Ig-like C2-type (immunoglobulin-like) domains.
In 23-week-old embryo found in epithelial podocytes of the periphery of mature and developing glomeruli.
Phosphorylated on tyrosine residues.
Cell membrane. Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane.
Zhang C et al. Autophagy is involved in mouse kidney development and podocyte differentiation regulated by Notch signalling. J Cell Mol Med21:1315-1328 (2017).
Read more (PubMed: 28158917) »
Guo H et al. Astragaloside IV protects against podocyte injury via SERCA2-dependent ER stress reduction and AMPKa-regulated autophagy induction in streptozotocin-induced diabetic nephropathy. Sci Rep7:6852 (2017).
Read more (PubMed: 28761152) »