製品の概要

  • 製品名Anti-Nephrin antibody
    Nephrin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Nephrin
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A 14 amino acid peptide from near the carboxy terminus of human Nephrin.

  • ポジティブ・コントロール
    • WB: HEK293 and Mouse kidney lysate. IHC: Mouse kidney tissue.
  • 特記事項Although there are publications stating this antibody works with Rat species in WB (PMID 19052104). The antibody however fail to stain the Rat kidney sections hence we have removed the species(from customer feedback). Further feedback using this antibody with Rat tissues would be very welcome

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 138 kDa).

ターゲット情報

  • 機能Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.
  • 組織特異性Specifically expressed in podocytes of kidney glomeruli.
  • 関連疾患Defects in NPHS1 are the cause of nephrotic syndrome type 1 (NPHS1) [MIM:256300]; also known as Finnish congenital nephrosis (CNF). A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure.
  • 配列類似性Belongs to the immunoglobulin superfamily.
    Contains 1 fibronectin type-III domain.
    Contains 8 Ig-like C2-type (immunoglobulin-like) domains.
  • 発生段階In 23-week-old embryo found in epithelial podocytes of the periphery of mature and developing glomeruli.
  • 翻訳後修飾Phosphorylated on tyrosine residues.
  • 細胞内局在Cell membrane. Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CNF antibody
    • Nephrin antibody
    • Nephrosis 1 congenital Finnish type antibody
    • Nephrosis 1, congenital, Finnish type (nephrin) antibody
    • NPHN antibody
    • NPHN_HUMAN antibody
    • NPHS 1 antibody
    • Nphs1 antibody
    • Renal glomerulus specific cell adhesion receptor antibody
    • Renal glomerulus-specific cell adhesion receptor antibody
    see all

Anti-Nephrin antibody 画像

  • All lanes : Anti-Nephrin antibody (ab58968)

    Lane 1 : HEK293 cell lysate
    Lane 2 : Mouse kidney lysate


    Predicted band size : 138 kDa
  • Anti-Nephrin antibody (ab58968) at 0.5 µg/ml + Mouse kidney tissue lysate at 56 µg with Milk at 5 %

    Secondary
    HRP conjugated Goat anti-rabbit IgG monoclonal at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 138 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab58968, at a concentration of 1 µg/ml, staining Nephrin in mouse kidney tissue by Immunohistochemistry.
  • All lanes : Anti-Nephrin antibody (ab58968) at 1 µg/ml

    Lane 1 : mouse kidney tissue lysate
    Lane 2 : mouse kidney tissue lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 138 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    The difference in MW size could be due to modification to the protein or just aberrant migration.

Anti-Nephrin antibody (ab58968) 使用論文

This product has been referenced in:
  • Li Z  et al. MiRNA expression profile and miRNA-mRNA integrated analysis (MMIA) during podocyte differentiation. Mol Genet Genomics 290:863-75 (2015). Read more (PubMed: 25433550) »
  • Denhez B  et al. Increased SHP-1 protein expression by high glucose levels reduces nephrin phosphorylation in podocytes. J Biol Chem 290:350-8 (2015). Read more (PubMed: 25404734) »

See all 16 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 56 µg
Gel Running Conditions Reduced Denaturing (4-20% Tris Glycin)
Sample Mouse Tissue lysate - whole (Kidney)
Specification Kidney
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 06 2013

Thank you for your email.
I am sorry to hear that these antibodies are not working as expected.
As for ab58968, we had replaced this antibody free of charge with ab85379. Since ab85379 was not tested in rat, sheep, and pig tissue, I had sent ...

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Thank you for your email. Please find below the tetsing discount code for ab85379. At the bottom of the email, I have listed the information regarding the free of charge replacement for ab15081 and ab58968.
DISCOUNT CODE: xxx
Expiration date:...

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Thank you for your reply and the additional information. This is very helpful to us for our internal
I have checked our stock, but we have, unfortunately, only a new lot for ab15081 - which I'd be happy to send you free of charge. For ab7671 and a...

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Thank you for contacting us.
I am sorry to hear that all 3 antibodies are not performing as expected.
Before proceeding with the free of charge replacements, could you please let me know the following experimental details:
1) What was th...

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Thank you for contacting us. I have checked with the originating lab and received the following reply:  "This has been brought to our attention a few times, and unfortunately, we do not have a good explanation.  It might be due to post-translationa...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (kidney)
Loading amount 10 µg
Specification kidney
Gel Running Conditions Non-reduced Denaturing (7.5%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Hongjie Wang

Verified customer

投稿 Mar 24 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"