Anti-kan 抗体 [4B4D1] (ab60018)
Key features and details
- Mouse monoclonal [4B4D1] to kan
- Suitable for: ELISA, WB
- Reacts with: Recombinant fragment
- Isotype: IgG2a
製品の概要
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製品名
Anti-kan antibody [4B4D1] -
製品の詳細
Mouse monoclonal [4B4D1] to kan -
由来種
Mouse -
アプリケーション
適用あり: ELISA, WBmore details -
種交差性
交差種: Recombinant fragment
交差が予測される動物種: Escherichia coli -
免疫原
Recombinant full length protein corresponding to kan.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.2 -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
4B4D1 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab60018の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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WB |
1/500 - 1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa).
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
WB
1/500 - 1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa). |
ターゲット情報
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関連性
Neomycin Phosphotransferase 2 (kan) confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids. -
参照データベース
- SwissProt: P14509 Escherichia coli
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別名
- Aminoglycoside 3' phosphotransferase antibody
- APH(3') II antibody
- APH(3')II antibody
see all
画像
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Anti-kan antibody [4B4D1] (ab60018) at 1/500 dilution + Recombinant kan expressed in E. coli.
Predicted band size: 29 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (11)
ab60018 は 11 報の論文で使用されています。
- Li X et al. Dehydrin MtCAS31 promotes autophagic degradation under drought stress. Autophagy N/A:1-16 (2019). PubMed: 31362589
- Kallehauge TB et al. Ribosome profiling-guided depletion of an mRNA increases cell growth rate and protein secretion. Sci Rep 7:40388 (2017). PubMed: 28091612
- Bentham MJ et al. NS2 is dispensable for efficient assembly of hepatitis C virus-like particles in a bipartite trans-encapsidation system. J Gen Virol 95:2427-41 (2014). PubMed: 25024280
- Holler CJ et al. Bridging integrator 1 (BIN1) protein expression increases in the Alzheimer's disease brain and correlates with neurofibrillary tangle pathology. J Alzheimers Dis 42:1221-7 (2014). PubMed: 25024306
- Wong TT et al. Production of zebrafish offspring from cultured female germline stem cells. PLoS One 8:e62660 (2013). IHC-FoFr, IHC - Wholemount ; Zebrafish . PubMed: 23671620