製品の概要

  • 製品名Anti-NENF antibody
    NENF 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NENF
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (Human) of 15 amino acids from near the C-terminus of NENF. (NP_037481).

  • ポジティブ・コントロール
    • Human kidney tissue lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab74474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 19 kDa).
ICC/IF Use a concentration of 20 µg/ml.

ターゲット情報

  • 機能Displays neurotrophic activity and activates phosphorylation of MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT in primary cultured neurons. Does not have mitogenic activity in primary cultured astrocytes. May play a role on neuronal differentiation and may have a transient effect on neural cell proliferation in neural precursor cells. Neurotrophic activity is enhanced by binding to heme.
  • 配列類似性Belongs to the cytochrome b5 family. MAPR subfamily.
    Contains 1 cytochrome b5 heme-binding domain.
  • ドメインThe cytochrome b5 heme-binding domain was proven to bind heme, although it lacks the conserved iron-binding His residue at position 82.
  • 細胞内局在Secreted > extracellular space.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cell growth inhibiting protein 47 antibody
    • Cell immortalization-related protein 2 antibody
    • CIR2 antibody
    • Nenf antibody
    • NENF_HUMAN antibody
    • Neudesin antibody
    • Neuron derived neurotrophic factor antibody
    • Neuron-derived neurotrophic factor antibody
    • SCIRP10 antibody
    • SCIRP10 related protein antibody
    • Secreted protein of unknown function antibody
    • Spinal cord injury related protein 10 antibody
    • SPUF antibody
    • SPUF protein antibody
    see all

Anti-NENF antibody 画像

  • Immunofluorescence of NENF in rat kidney tissue with ab74474 at 20 ug/mL.

  • Lane 1 : Anti-NENF antibody (ab74474) at 1 µg/ml
    Lane 2 : Anti-NENF antibody (ab74474) at 2 µg/ml

    Lane 1 : Human kidney tissue lysate
    Lane 2 : Human kidney tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 19 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • ab74474 at 10 µg/ml staining NENF in rat kidney tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

Anti-NENF antibody (ab74474) 使用論文

This product has been referenced in:
  • Stefanska B  et al. Genome-wide study of hypomethylated and induced genes in patients with liver cancer unravels novel anticancer targets. Clin Cancer Res 20:3118-32 (2014). WB ; Human . Read more (PubMed: 24763612) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab74474.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"