製品の概要

  • 製品名Anti-NELFe antibody
    NELFe 一次抗体 製品一覧
  • 製品の詳細
    Sheep polyclonal to NELFe
  • アプリケーション適用あり: ICC/IF, IP, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Fusion protein (Human)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/800.
IP Use at an assay dependent dilution.
WB 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).

ターゲット情報

  • 機能Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.
  • 組織特異性Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.
  • 配列類似性Belongs to the RRM NELF-E family.
    Contains 1 RRM (RNA recognition motif) domain.
  • ドメインThe RRM domain interacts with RNA, and is essential for NELF complex function. It is however not required for the NELF complex formation.
  • 翻訳後修飾Sumoylated.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Major histocompatibility complex gene RD antibody
    • Negative elongation factor E antibody
    • Negative elongation factor polypeptide E antibody
    • NELF e antibody
    • NELF-E antibody
    • Nelfe antibody
    • NELFE_HUMAN antibody
    • Nuclear protein antibody
    • RD antibody
    • RD protein antibody
    • RD RNA binding protein antibody
    • RDP antibody
    • RNA-binding protein RD antibody
    see all

Anti-NELFe antibody 画像

  • All lanes : Anti-NELFe antibody (ab28038) at 1/1000 dilution

    Lane 1 : NELFe-GST fusion protein
    Lane 2 : HeLa cell nuclear extract

    Secondary
    donkey anti-sheep peroxidase linked at 1/20000 dilution

    Predicted band size : 43 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • HeLa cells stained with: 1. HCM antibody ab28038 diluted 1/800 and donkey anti-sheep Alexa 647 secondary antibody diluted 1/1000 2. DAPI

Anti-NELFe antibody (ab28038) 使用論文

ab28038 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"