製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to NEK7
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Orangutan
  • 免疫原

    A region between residue 1 and 50 of human NEK7 (NP_598001.1)

  • ポジティブ・コントロール
    • HeLa and 293T cell lysates

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab95873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 35 kDa.
IP Use at 5-15 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 組織特異性
    Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.
  • 配列類似性
    Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EC 2.7.11.1 antibody
    • NEK7 antibody
    • NEK7_HUMAN antibody
    • Never in mitosis A-related kinase 7 antibody
    • Never in mitosis gene a-related kinase 7 antibody
    • NIMA (never in mitosis gene a) related kinase 7 antibody
    • NIMA (never in mitosis gene a)-related expressed kinase 7 antibody
    • NimA related protein kinase 7 antibody
    • NIMA-related kinase 7 antibody
    • NimA-related protein kinase 7 antibody
    • Serine/threonine-protein kinase Nek7 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling NEK7 with ab95873 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-NEK7 antibody (ab95873) at 0.4 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg


    Predicted band size : 35 kDa
  • Detection of NEK7 by Western Blot of Immunprecipitate. (1) ab95873 at 0.4µg/ml staining NEK7 which had been immunoprecipitated from HeLa whole cell lysate using ab95873 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane), (2) control IgG. Detection: Chemiluminescence with exposure time of 30 seconds.

参考文献

ab95873 has not yet been referenced specifically in any publications.

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