製品の概要

  • 製品名Anti-NEK7 antibody
    NEK7 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NEK7
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Saccharomyces cerevisiae, Caenorhabditis elegans, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (DAKARADCIK EIDLLKQLNH PNVIKYYASF IEDNELNIVL ELADAGDLSR) of Human NEK7 (NP_598001).

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab80948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

ターゲット情報

Anti-NEK7 antibody 画像

  • Anti-NEK7 antibody (ab80948) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 12%
  • All lanes : Anti-NEK7 antibody (ab80948) at 1/2000 dilution

    Lane 1 : Mouse brain tissue cytoplasmic lysate
    Lane 2 : Mouse brain tissue cytoplasmic lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 35,38 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds

    This image is courtesy of an Abreview submitted by Dr Maryna Polyakova

    Blocked with 5% non-fat Milk for 1 hour at 18°C

     

    Secondary antibody - anti-rabbit HRP (ab6721)

    See Abreview

Anti-NEK7 antibody (ab80948) 使用論文

ab80948 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry free floating
Sample Mouse Tissue sections (Brain Coronal sections)
Specification Brain Coronal sections
Username

Abcam user community

Verified customer

投稿 Apr 15 2016

Application Western blot
Sample Mouse Tissue lysate - other (brain tissue lysate, cytosolic fraction)
Loading amount 10 µg
Specification brain tissue lysate, cytosolic fraction
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Dr. Maryna Polyakova

Verified customer

投稿 May 15 2012

Thank you for your reply.
Reviewing the remaining antibodies that are already tested in WB and the species you wish to use, the following do not yet have a western blot image, and I would be pleased to arrange a testing discount for these addition...

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Thank you very much for your interest in our antibodies.

We appreciate receiving feedback and data from customers, and we do have a testing discount offer available.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"