製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to NEK7
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-302 of Human NEK7

  • ポジティブ・コントロール
    • NEK7 transfected mammalian cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 34 kDa).

ターゲット情報

  • 組織特異性
    Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.
  • 配列類似性
    Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EC 2.7.11.1 antibody
    • NEK7 antibody
    • NEK7_HUMAN antibody
    • Never in mitosis A-related kinase 7 antibody
    • Never in mitosis gene a-related kinase 7 antibody
    • NIMA (never in mitosis gene a) related kinase 7 antibody
    • NIMA (never in mitosis gene a)-related expressed kinase 7 antibody
    • NimA related protein kinase 7 antibody
    • NIMA-related kinase 7 antibody
    • NimA-related protein kinase 7 antibody
    • Serine/threonine-protein kinase Nek7 antibody
    see all

画像

  • All lanes : Anti-NEK7 antibody (ab68060) at 1/500 dilution

    Lane 1 : NEK7 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 34 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

This product has been referenced in:
  • de Souza EE  et al. Characterization of the human NEK7 interactome suggests catalytic and regulatory properties distinct from those of NEK6. J Proteome Res 13:4074-90 (2014). Read more (PubMed: 25093993) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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