製品の概要

  • 製品名Anti-NEK4 antibody
    NEK4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NEK4
  • アプリケーション適用あり: IHC-P, IPmore details
    適用なし: WB
  • 種交差性
    交差種: Human
    交差が予測される動物種: Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 350-400 (KQDLSNTTEL ATISSVNIDI LPAKGRDSVS DGFVQENQPR YLDASNELGG I) of Human NEK4 (NP_003148.2).

  • ポジティブ・コントロール
    • HeLa whole cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab95189 was affinity purified using an epitope specific to NEK4 immobilized on solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab95189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 関連性NEK4 (NIMA related kinase 4) is a serine/threonine protein kinase related to the asperigillus NIMA (never in mitosis gene a) kinase. NEK4 is proposed to play a role in cell division. Highest expression in adult heart, followed by pancreas, skeletal muscle, brain, liver, kidney, lung and placenta. Present in most primary carcinomas. There are two named isoforms produced by alternative splicing.
    • 細胞内局在Nuclear
    • 参照データベース
    • 別名
      • EC 2.7.11.1 antibody
      • MGC33171 antibody
      • Never in mitosis A related kinase 4 antibody
      • Never in mitosis gene A related kinase 4 antibody
      • NIMA (never in mitosis gene a) related kinase 4 antibody
      • NIMA (never in mitosis gene a)-related expressed kinase 4 antibody
      • NIMA related kinase 4 antibody
      • NimA related protein kinase 4 antibody
      • NLK2 antibody
      • NRK2 antibody
      • OTTHUMP00000213582 antibody
      • Pp12301 antibody
      • Serine/threonine kinase 2 antibody
      • Serine/threonine protein kinase 2 antibody
      • Serine/threonine protein kinase Nek4 antibody
      • Serine/threonine protein kinase NRK2 antibody
      • STK2 antibody
      see all

    Anti-NEK4 antibody 画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling NEK4 with ab95189 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of NEK4 in immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab95189 at 3 µg/mg lysate for IP. An anti-NEK4 antibody, which recognizes a downstream epitope, was used at 1µg/ml for the subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 30 seconds.

    Anti-NEK4 antibody (ab95189) 使用論文

    ab95189 has not yet been referenced specifically in any publications.

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