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RabMAb

Anti-NEK2 抗体 [EPR3787] (ab109283)

製品の概要

  • 製品名
    Anti-NEK2 antibody [EPR3787]
    NEK2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3787] to NEK2
  • アプリケーション
    適用あり: WBmore details
    適用なし: Flow Cyt,ICC,IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human NEK2

  • ポジティブ・コントロール
    • Jurkat, 293T and K562 cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • ターゲット情報

    • 機能
      Protein kinase that is involved in mitotic regulation. Integral component of the mitotic spindle-assembly checkpoint which is necessary for proper chromosome segregation during metaphase-anaphase transition. Required for association of MAD2L1 to kinetochore. Phosphorylates SGOL1. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.
    • 組織特異性
      Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types.
    • 配列類似性
      Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
      Contains 1 protein kinase domain.
    • 発生段階
      Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Isoform 1 and isoform 2 expression is low in the G1 phase and increases in the S/G2 phases. Isoform 1 is absent from cells arrested in the G2/M prometaphase. Isoform 2 remains present in cells arrested in the G2/M prometaphase.
    • 翻訳後修飾
      It is unsure whether Thr-170 or Ser-171 is phosphorylated.
    • 細胞内局在
      Cytoplasm. Predominantly cytoplasmic; Nucleus > nucleolus. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end and Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Co-localizes with SGOL1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope.
    • Information by UniProt
    • 参照データベース
    • 別名
      • HSPK 21 antibody
      • HsPK21 antibody
      • NEK 2 antibody
      • NEK2 antibody
      • NEK2_HUMAN antibody
      • NEK2A antibody
      • Never in mitosis A-related kinase 2 antibody
      • NIMA (never in mitosis gene a) related kinase 2 antibody
      • NimA like protein kinase 1 antibody
      • NIMA related kinase 2 antibody
      • NimA related protein kinase 2 antibody
      • NimA-like protein kinase 1 antibody
      • NimA-related protein kinase 2 antibody
      • NLK 1 antibody
      • NLK1 antibody
      • Serine/threonine-protein kinase Nek2 antibody
      see all

    画像

    • All lanes : Anti-NEK2 antibody [EPR3787] (ab109283) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 52 kDa

    プロトコール

    参考文献

    ab109283 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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