製品の概要

  • 製品名Anti-NEK2 antibody [EPR3787]
    NEK2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3787] to NEK2
  • アプリケーション適用あり: WBmore details
    適用なし: Flow Cyt,ICC,IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human NEK2

  • ポジティブ・コントロール
    • Jurkat, 293T and K562 cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
  • 追加情報Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • ターゲット情報

    • 機能Protein kinase that is involved in mitotic regulation. Integral component of the mitotic spindle-assembly checkpoint which is necessary for proper chromosome segregation during metaphase-anaphase transition. Required for association of MAD2L1 to kinetochore. Phosphorylates SGOL1. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.
    • 組織特異性Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types.
    • 配列類似性Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
      Contains 1 protein kinase domain.
    • 発生段階Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Isoform 1 and isoform 2 expression is low in the G1 phase and increases in the S/G2 phases. Isoform 1 is absent from cells arrested in the G2/M prometaphase. Isoform 2 remains present in cells arrested in the G2/M prometaphase.
    • 翻訳後修飾It is unsure whether Thr-170 or Ser-171 is phosphorylated.
    • 細胞内局在Cytoplasm. Predominantly cytoplasmic; Nucleus > nucleolus. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end and Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Co-localizes with SGOL1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope.
    • Information by UniProt
    • 参照データベース
    • 別名
      • HSPK 21 antibody
      • HsPK21 antibody
      • NEK 2 antibody
      • NEK2 antibody
      • NEK2_HUMAN antibody
      • NEK2A antibody
      • Never in mitosis A-related kinase 2 antibody
      • NIMA (never in mitosis gene a) related kinase 2 antibody
      • NimA like protein kinase 1 antibody
      • NIMA related kinase 2 antibody
      • NimA related protein kinase 2 antibody
      • NimA-like protein kinase 1 antibody
      • NimA-related protein kinase 2 antibody
      • NLK 1 antibody
      • NLK1 antibody
      • Serine/threonine-protein kinase Nek2 antibody
      see all

    Anti-NEK2 antibody [EPR3787] 画像

    • All lanes : Anti-NEK2 antibody [EPR3787] (ab109283) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 52 kDa

    Anti-NEK2 antibody [EPR3787] (ab109283) 使用論文

    ab109283 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"