製品の概要

  • 製品名Anti-NEK2 antibody
    NEK2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to NEK2
  • アプリケーション適用あり: IHC-P, WB, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 331-445 of Human NEK2

  • ポジティブ・コントロール
    • ICC/IF: A549 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Place sample in 1X citrate buffer (pH 6.0) and microwave at 750W for 20 minutes, cool sample subsequently.
WB Use a concentration of 1 - 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Protein kinase that is involved in mitotic regulation. Integral component of the mitotic spindle-assembly checkpoint which is necessary for proper chromosome segregation during metaphase-anaphase transition. Required for association of MAD2L1 to kinetochore. Phosphorylates SGOL1. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.
  • 組織特異性Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types.
  • 配列類似性Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
  • 発生段階Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Isoform 1 and isoform 2 expression is low in the G1 phase and increases in the S/G2 phases. Isoform 1 is absent from cells arrested in the G2/M prometaphase. Isoform 2 remains present in cells arrested in the G2/M prometaphase.
  • 翻訳後修飾It is unsure whether Thr-170 or Ser-171 is phosphorylated.
  • 細胞内局在Cytoplasm. Predominantly cytoplasmic; Nucleus > nucleolus. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end and Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Co-localizes with SGOL1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HSPK 21 antibody
    • HsPK21 antibody
    • NEK 2 antibody
    • NEK2 antibody
    • NEK2_HUMAN antibody
    • NEK2A antibody
    • Never in mitosis A-related kinase 2 antibody
    • NIMA (never in mitosis gene a) related kinase 2 antibody
    • NimA like protein kinase 1 antibody
    • NIMA related kinase 2 antibody
    • NimA related protein kinase 2 antibody
    • NimA-like protein kinase 1 antibody
    • NimA-related protein kinase 2 antibody
    • NLK 1 antibody
    • NLK1 antibody
    • Serine/threonine-protein kinase Nek2 antibody
    see all

Anti-NEK2 antibody 画像

  • ICC/IF image of ab55550 stained A549 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55550, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • NEK2 antibody (ab55550) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human testis.
  • Western blot against tagged recombinant protein immunogen using ab55550 NEK2 antibody at 1ug/ml. Predicted band size of immunogen is 39 kDa
  • Overlay histogram showing Jurkat cells stained with ab55550 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55550, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-NEK2 antibody (ab55550) 使用論文

This product has been referenced in:
  • Stricker TP  et al. Expression profiling of 519 kinase genes in matched malignant peripheral nerve sheath tumor/plexiform neurofibroma samples is discriminatory and identifies mitotic regulators BUB1B, PBK and NEK2 as overexpressed with transformation. Mod Pathol N/A:N/A (2013). Read more (PubMed: 23370767) »

See 1 Publication for this product

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mIMCD-3 cells)
Specification mIMCD-3 cells
Fixative Methanol
Permeabilization Yes - NP40
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Mr. Benjamin Tyrrell

Verified customer

投稿 Aug 26 2009

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample African Green Monkey Cell lysate - whole cell (mIMCD-3 Cell lysate)
Loading amount 100 µg
Specification mIMCD-3 Cell lysate
Gel Running Conditions Reduced Denaturing (12)
Username

Mr. Benjamin Tyrrell

Verified customer

投稿 Aug 26 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"