製品の概要

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris-saline. pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab14753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
WB
  • 追加情報
    Tested in peptide ELISA: antibody detection limit dilution 1:16,000.
    IHC-P: Use at a concentration of 5 µg/ml.
    Western Blot: preliminary experiments give ~60kDa band in human liver and brain lysates at 1ug/ml. However predicted band size = 44kDa (NP_078884)

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches.
    • 組織特異性
      Ubiquitous.
    • 配列類似性
      Belongs to the FPG family.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DNA (apurinic or apyrimidinic site) lyase Neil1 antibody
      • DNA glycosylase/AP lyase Neil 1 antibody
      • DNA glycosylase/AP lyase Neil1 antibody
      • DNA-(apurinic or apyrimidinic site) lyase Neil1 antibody
      • Endonuclease 8 like 1 antibody
      • Endonuclease 8-like 1 antibody
      • Endonuclease VIII antibody
      • Endonuclease VIII like 1 antibody
      • Endonuclease VIII-like 1 antibody
      • FLJ22402 antibody
      • FPG1 antibody
      • hFPG1 antibody
      • NEH 1 antibody
      • NEH1 antibody
      • NEI 1 antibody
      • Nei endonuclease VIII like 1 (E. coli) antibody
      • Nei endonuclease VIII like 1 antibody
      • Nei homolog 1 antibody
      • Nei like 1 antibody
      • Nei like protein 1 antibody
      • Nei-like protein 1 antibody
      • NEI1 antibody
      • NEIL 1 antibody
      • Neil1 antibody
      • NEIL1_HUMAN antibody
      see all

    画像

    • ab14753 at 5ug/ml staining NEIL1 in human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). A AP-staining procedure was used for detection.

    参考文献

    ab14753 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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