製品の概要

  • 製品名Anti-NEDD8 antibody
    NEDD8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NEDD8
  • アプリケーション適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    KLH-conjugated synthetic peptide selected within aa 1-100 of human NEDD8.

  • ポジティブ・コントロール
    • HeLa cell lysates and human hepatocarcinoma tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーPreservative: 0.09% None
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab38634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
WB 1/100 - 1/500. Predicted molecular weight: 9 kDa.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
  • 組織特異性Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
  • 配列類似性Belongs to the ubiquitin family.
  • 翻訳後修飾Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.
  • 細胞内局在Nucleus. Mainly nuclear.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ43224 antibody
    • MGC104393 antibody
    • MGC125896 antibody
    • MGC125897 antibody
    • NED8 antibody
    • NEDD 8 antibody
    • NEDD-8 antibody
    • Nedd8 antibody
    • NEDD8_HUMAN antibody
    • Neddylin antibody
    • Neural precursor cell expressed developmentally down regulated 8 antibody
    • Neural precursor cell expressed developmentally down regulated gene 8 antibody
    • Neural precursor cell expressed developmentally down-regulated protein 8 antibody
    • Rub1 antibody
    • Ubiquitin like protein Nedd 8 antibody
    • Ubiquitin like protein Nedd8 antibody
    • Ubiquitin-like protein Nedd8 antibody
    see all

Anti-NEDD8 antibody 画像

  • Anti-NEDD8 antibody (ab38634) at 1/100 dilution + HeLa cell lysates

    Predicted band size : 9 kDa
    Observed band size : 10 kDa (why is the actual band size different from the predicted?)
  • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-NEDD8 antibody (ab38634) 使用論文

This product has been referenced in:
  • Eckle T  et al. Adora2b-elicited Per2 stabilization promotes a HIF-dependent metabolic switch crucial for myocardial adaptation to ischemia. Nat Med 18:774-82 (2012). Read more (PubMed: 22504483) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (12)
Sample Human Cell lysate - whole cell (mcf 7)
Specification mcf 7
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Mr. Sanjib Chaudhary

Verified customer

投稿 Aug 12 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk McManus

Verified customer

投稿 Jun 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"