Anti-NEDD4-2 (phospho S328) 抗体 (ab95399)
Key features and details
- Rabbit polyclonal to NEDD4-2 (phospho S328)
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-NEDD4-2 (phospho S328) antibody
NEDD4-2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NEDD4-2 (phospho S328) -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide corresponding to Mouse NEDD4-2 conjugated to keyhole limpet haemocyanin.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab95399の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent concentration.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2. -
組織特異性
Ubiquitously expressed, with highest levels in prostate, pancreas and kidney. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 1 C2 domain.
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 4 WW domains. -
翻訳後修飾
Phosphorylated by SGK1 or PKA; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation.
Auto-ubiquitinated. -
細胞内局在
Cytoplasm. May be recruited to exosomes by NDFIP1. - Information by UniProt
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参照データベース
- Entrez Gene: 83814 Mouse
- SwissProt: Q8CFI0 Mouse
- Unigene: 98668 Mouse
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別名
- E3 ubiquitin protein ligase NEDD4 like protein antibody
- E3 ubiquitin-protein ligase NEDD4-like antibody
- KIAA0439 antibody
see all
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (4)
ab95399 は 4 報の論文で使用されています。
- Deftu AF et al. The Antidiabetic Drug Metformin Regulates Voltage-Gated Sodium Channel NaV1.7 via the Ubiquitin-Ligase NEDD4-2. eNeuro 9:N/A (2022). PubMed: 35131865
- Saha B et al. WNK1 is a chloride-stimulated scaffold that regulates mTORC2 activity and ion transport. J Cell Sci 135:N/A (2022). PubMed: 36373794
- Gleason CE et al. mTORC2 regulates renal tubule sodium uptake by promoting ENaC activity. J Clin Invest 125:117-28 (2015). PubMed: 25415435
- Caohuy H et al. Activation of 3-phosphoinositide-dependent kinase 1 (PDK1) and serum- and glucocorticoid-induced protein kinase 1 (SGK1) by short-chain sphingolipid C4-ceramide rescues the trafficking defect of ?F508-cystic fibrosis transmembrane conductance regulator (?F508-CFTR). J Biol Chem 289:35953-68 (2014). PubMed: 25384981