製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Nectin 3
  • アプリケーション
    適用あり: IHC-Fr, WBmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to a region surrounding amino acid 536 of mouse nectin 3

  • ポジティブ・コントロール
    • 3T3 cell lysate; Rat kidney lysate

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 30% Glycerol, 0.5% BSA, PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab63931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. See Abreview.
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).

ターゲット情報

  • 機能
    Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.
  • 組織特異性
    Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.
  • 配列類似性
    Belongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD113 antibody
    • CDw113 antibody
    • DKFZP566B0846 antibody
    • FLJ90624 antibody
    • Nectin-3 antibody
    • Nectin3 antibody
    • Poliovirus receptor related 3 antibody
    • Poliovirus receptor related protein 3 antibody
    • Poliovirus receptor-like 3 antibody
    • Poliovirus receptor-related protein 3 antibody
    • PPR3 antibody
    • PRR3 antibody
    • Pvrl3 antibody
    • PVRL3_HUMAN antibody
    • PVRR3 antibody
    see all

Anti-Nectin 3 antibody 画像

  • All lanes : Anti-Nectin 3 antibody (ab63931) at 1 µg

    Lane 1 : Rat kidney lysate
    Lane 2 : 3T3 cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 83 kDa
    Observed band size : 83 kDa

Anti-Nectin 3 antibody (ab63931) 使用論文

This product has been referenced in:
  • Li J  et al. Differential Behavioral and Neurobiological Effects of Chronic Corticosterone Treatment in Adolescent and Adult Rats. Front Mol Neurosci 10:25 (2017). WB . Read more (PubMed: 28210212) »
  • Nakata H  et al. Quantitative analysis of the cellular composition in seminiferous tubules in normal and genetically modified infertile mice. J Histochem Cytochem 63:99-113 (2015). Read more (PubMed: 25411188) »

See all 10 Publications for this product

Product Wall

We have previously been in contact regarding ab63931, which was not providing satisfactory results.

We provided some troubleshooting tips for the protocol, and I am writing to inquire if these suggestions have helped to improve the results...

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Thank you for your reply.

What I wanted to say is that the HEK293 cells do also have Nectin-3 expression. The antibody ab63931 is likely to recognize also the Nectin-3 isoforms expressed by the human cell. Therefore it could be that the obser...

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Thank you for providing these additional details and your patience.

The laboratory has confirmed that the immunogen for thisantibody isa synthetic peptide corresponding to residues surrounding amino acids 536 of mouse Nectin-3 and that this r...

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Thank you for your patience.

Unfortunately we can not provide the exact immunogen sequence for this antibody as it is proprietary information of the laboratory.

As said, please provide us with more information about the experiment. ...

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Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. I have contacted the laboratory re...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole C57BL/6 mouse hippocampus)
Loading amount
40 µg
Specification
Whole C57BL/6 mouse hippocampus
Gel Running Conditions
Non-reduced Denaturing (NuPAGE® 10% Bis-Tris gel (Invitrogen))
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Oct 30 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Hippocampus)
Specification
Hippocampus
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Dec 17 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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