製品の概要

  • 製品名
    Anti-Nectin 3 antibody [103-A1]
    Nectin 3 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [103-A1] to Nectin 3
  • アプリケーション
    適用あり: Functional Studies, ICC/IF, Flow Cyt, IHC-Fr, IPmore details
    適用なし: WB
  • 種交差性
    交差種: Mouse
    非交差種: Rat, Human
  • 免疫原

    Recombinant fragment, corresponding to Mouse Nectin 3 extracellular domain, amino acids 50-400 fused to IgG Fc.

  • ポジティブ・コントロール
    • L-cells (A cultured line of C3H mouse fibroblasts)

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    Provided as a 0.2µm purified antibody solution
  • ポリ/モノ
    モノクローナル
  • クローン名
    103-A1
  • アイソタイプ
    IgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab16913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 機能
      Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.
    • 組織特異性
      Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.
    • 配列類似性
      Belongs to the nectin family.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • 細胞内局在
      Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD113 antibody
      • CDw113 antibody
      • DKFZP566B0846 antibody
      • FLJ90624 antibody
      • Nectin-3 antibody
      • Nectin3 antibody
      • Poliovirus receptor related 3 antibody
      • Poliovirus receptor related protein 3 antibody
      • Poliovirus receptor-like 3 antibody
      • Poliovirus receptor-related protein 3 antibody
      • PPR3 antibody
      • PRR3 antibody
      • Pvrl3 antibody
      • PVRL3_HUMAN antibody
      • PVRR3 antibody
      see all

    参考文献

    This product has been referenced in:
    • Lachke SA  et al. The cell adhesion gene PVRL3 is associated with congenital ocular defects. Hum Genet 131:235-50 (2012). IHC-Fr ; Mouse . Read more (PubMed: 21769484) »
    • Tanaka-Okamoto M  et al. Increased susceptibility to spontaneous lung cancer in mice lacking LIM-domain only 7. Cancer Sci 100:608-16 (2009). Read more (PubMed: 19215226) »

    See all 3 Publications for this product

    レビューと Q&A

    Thank you for contacting Abcam.

    According to our notes, ab16913 is not suitable for western blotting. However the Nectin3 antibodies listed below, are suitable for use in western blotting:

    http://www.abcam.com/Nectin-3-antibody-ab6393...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Embryonic stem cells)
    Specification
    Embryonic stem cells
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    投稿 May 10 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Mouse Cell (Embryonic stem cells)
    Specification
    Embryonic stem cells
    Preparation
    Cell harvesting/tissue preparation method: Trypsination
    Sample buffer: PBS, 4%FCS
    Permeabilization
    No
    Gating Strategy
    Debris was gated away by FSC/SSC and dead cells by PI.
    Username

    Abcam user community

    Verified customer

    投稿 May 09 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (testis)
    Specification
    testis
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Username

    Y Toyama

    Verified customer

    投稿 Aug 25 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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