製品の概要

  • 製品名
    Anti-Nectin 2 antibody [B-C12]
    Nectin 2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [B-C12] to Nectin 2
  • 特異性
    ab47171 recognises the Nectin-2, the polio retrovirus receptor-2 (PRR2)
  • アプリケーション
    適用あり: IHC-Fr, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    KG-1 cell line

  • ポジティブ・コントロール
    • KG-1 cell line

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Ion Exchange Chromatography
  • 特記事項(精製)
    Sterile-filtered through 0.22 µm and treated to remove endotoxins. Carrier and preservative free
  • ポリ/モノ
    モノクローナル
  • クローン名
    B-C12
  • ミエローマ
    x63-Ag8.653
  • アイソタイプ
    IgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab47171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr
ICC/IF
Flow Cyt
  • 追加情報
    Flow Cyt: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    ICC/IF: Use at a concentration of 1-10 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Probable cell adhesion protein.
    • 組織特異性
      Ubiquitous.
    • 配列類似性
      Belongs to the nectin family.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • 細胞内局在
      Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD 112 antibody
      • CD112 antibody
      • CD112 antigen antibody
      • Herpes virus entry mediator B antibody
      • Herpes virus entry protein B antibody
      • Herpesvirus entry mediator B antibody
      • Herpesvirus entry protein B antibody
      • Hve B antibody
      • HveB antibody
      • Nectin-2 antibody
      • Nectin2 antibody
      • Poliovirus receptor like 2 antibody
      • Poliovirus receptor related 2 (herpesvirus entry mediator B) antibody
      • Poliovirus receptor related 2 antibody
      • Poliovirus receptor related protein 2 antibody
      • Poliovirus receptor-related protein 2 antibody
      • PRR 2 antibody
      • PRR2 antibody
      • PVRL 2 antibody
      • PVRL2 antibody
      • PVRL2_HUMAN antibody
      • PVRR 2 antibody
      • PVRR2 antibody
      see all

    画像

    • A typical staining pattern with ab47171 monoclonal antibody of KG-1 cell line
    • ICC/IF image of ab47171 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47171, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    参考文献

    This product has been referenced in:
    • Ogbomo H  et al. Myxoma virus infection promotes NK lysis of malignant gliomas in vitro and in vivo. PLoS One 8:e66825 (2013). Flow Cyt ; Human . Read more (PubMed: 23762498) »

    See 1 Publication for this product

    レビューと Q&A

    There are currently no Customer reviews or Questions for ab47171.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録