製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Necdin
  • アプリケーション
    適用あり: WB, ChIPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Fusion protein (GST-tag) corresponding to Human Necdin aa 1-143 (N terminal).

  • ポジティブ・コントロール
    • Jurkat lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: 30% Glycerol, 0.15M Sodium chloride, 0.1M Tris glycine. pH 7.4
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab18554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa). Non-specific proteins were also detected at 70 and 100kDa, detection of which was inhibited by preincubation with the immunizing peptide.
ChIP Use at an assay dependent dilution. PubMed: 20663865

ターゲット情報

  • 機能
    Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle-promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences.
  • 組織特異性
    Almost ubiquitous. Detected in fetal brain, lung, liver and kidney; in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Not detected in peripheral blood leukocytes. In brain, restricted to post-mitotic neurons.
  • 配列類似性
    Contains 1 MAGE domain.
  • 細胞内局在
    Perikaryon. Nucleus. Neural perikarya, translocates to the nucleus of postmitotic neurons and interacts with the nuclear matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HsT16328 antibody
    • NDN antibody
    • NECD_HUMAN antibody
    • Necdin antibody
    • Necdin homolog antibody
    • Necdin MAGE family member antibody
    • necdin related protein antibody
    • PWCR antibody
    see all

Anti-Necdin antibody 画像



  • Predicted band size : 36 kDa

Anti-Necdin antibody (ab18554) 使用論文

This product has been referenced in:
  • Wu M  et al. The ciliary protein cystin forms a regulatory complex with necdin to modulate Myc expression. PLoS One 8:e83062 (2013). ChIP . Read more (PubMed: 24349431) »
  • Haviland R  et al. Necdin, a Negative Growth Regulator, Is a Novel STAT3 Target Gene Down-Regulated in Human Cancer. PLoS One 6:e24923 (2011). WB ; Human . Read more (PubMed: 22046235) »

See all 5 Publications for this product

Product Wall

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issuedtwo free of charge replacements:

One vial of ab18554 is being replaced with one vial of ab9574.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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