製品の概要

  • 製品名Anti-Ndufs1 antibody
    Ndufs1 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to Ndufs1
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken, Cow, Xenopus laevis, Chimpanzee, Zebrafish
  • 免疫原

    Fusion protein:

    TNSEKSKKAREGVMEFLLANHPLDCPICDQGGECDLQDQSMMFGSDRSRF LEGKRAVEDKNIGPLVKTIMTRCIQCTRCIRFASEIAGVDDLGTTGRGND

    , corresponding to amino acids 104/203 of Mouse Ndufs1

  • 特記事項Produced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab22094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 79 kDa.
ICC/IF Use at an assay dependent dilution. PubMed: 20966424

ターゲット情報

  • 機能Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized.
  • 関連疾患Defects in NDUFS1 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
  • 配列類似性Belongs to the complex I 75 kDa subunit family.
    Contains 1 2Fe-2S ferredoxin-type domain.
  • 細胞内局在Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CI-75kD antibody
    • Complex I 75Kd antibody
    • Complex I, mitochondrial respiratory chain, 75 kD subunit antibody
    • Complex I-75kD antibody
    • mitochondrial antibody
    • NADH coenzyme Q reductase antibody
    • NADH dehydrogenase (ubiquinone) FeS protein 1 (75kD) (NADH coenzyme Q reductase) antibody
    • NADH ubiquinone oxidoreductase 75 kDa subunit mitochondrial antibody
    • NADH-ubiquinone oxidoreductase 75 kDa subunit antibody
    • NDUFS1 antibody
    • NDUS1_HUMAN antibody
    • PRO1304 antibody
    see all

Anti-Ndufs1 antibody 画像

  • All lanes : Anti-Ndufs1 antibody (ab22094) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 79 kDa

Anti-Ndufs1 antibody (ab22094) 使用論文

This product has been referenced in:
  • Suryawanshi AR  et al. Differential Proteomics Leads to Identification of Domain Specific Epididymal Sperm Proteins. J Androl : (2010). WB, ICC/IF ; Human, Rat . Read more (PubMed: 20966424) »

See 1 Publication for this product

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Grazie per la sua richiesta.

Non abbiamo l'anticorpo secondarioesattonel nostro catalogo che era utilizzato nel Western blot sulla scheda tecnica. Purtroppo posso confermare che il ab22094 è un anticorpo policlonale del topo che sar&ag...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"