製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to NDUFB9
  • 特異性
    ab106699 is predicted not to cross-react with other Complex I LYR family members
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A 17 amino acid synthetic peptide near the carboxy terminus of Human NDUFB9.

  • ポジティブ・コントロール
    • Human liver tissue lysate and Rat liver tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab106699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 22 kDa.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.

ターゲット情報

  • 機能
    Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • 配列類似性
    Belongs to the complex I LYR family.
  • 細胞内局在
    Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B22 antibody
    • CI B22 antibody
    • CI-B22 antibody
    • complex I B22 subunit antibody
    • Complex I-B22 antibody
    • DKFZp566O173 antibody
    • FLJ22885 antibody
    • I B22 antibody
    • LYR motif containing protein 3 antibody
    • LYR motif-containing protein 3 antibody
    • LYRM3 antibody
    • NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa antibody
    • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 antibody
    • NADH ubiquinone oxidoreductase B22 subunit antibody
    • NADH-ubiquinone oxidoreductase B22 subunit antibody
    • NDUB9_HUMAN antibody
    • Ndufb9 antibody
    • UQOR22 antibody
    see all

画像

  • Immunofluorescence of NDUFB9 in rat liver tissue with ab106699 at 20 ug/mL.

  • Lane 1 : Anti-NDUFB9 antibody (ab106699) at 1 µg/ml
    Lane 2 : Anti-NDUFB9 antibody (ab106699) at 2 µg/ml

    Lane 1 : Human liver tissue lysate
    Lane 2 : Human liver tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 22 kDa
  • ab106699, at 5 µg/ml, staining NDUFB9 by immunohistochemistry

参考文献

This product has been referenced in:
  • Miwa S  et al. Low abundance of the matrix arm of complex I in mitochondria predicts longevity in mice. Nat Commun 5:3837 (2014). WB ; Mouse . Read more (PubMed: 24815183) »

See 1 Publication for this product

レビューと Q&A

Application
Western blot
Sample
Mouse Cell lysate - other (AML12 hepatocytes, mitochondrial fraction)
Gel Running Conditions
Reduced Denaturing (18)
Loading amount
25 µg
Specification
AML12 hepatocytes, mitochondrial fraction
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Oct 13 2017

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
American Robin (Turdis migratorius) Cell lysate - other (fibroblasts, mitochondrial fraction)
Gel Running Conditions
Reduced Denaturing (18)
Loading amount
25 µg
Specification
fibroblasts, mitochondrial fraction
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Sep 12 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Drosophila melanogaster Cell lysate - other (whole fly mitochondrial fraction)
Gel Running Conditions
Reduced Denaturing (18)
Loading amount
25 µg
Specification
whole fly mitochondrial fraction
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Aug 31 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録