製品の概要

  • 製品名Anti-NDUFB6 antibody [21C11BC11]
    NDUFB6 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [21C11BC11] to NDUFB6
  • アプリケーション適用あり: Flow Cyt, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 免疫原

    NDUFB6 from cow

  • ポジティブ・コントロール
    • Isolated mitochondria from Human heart, bovine heart, rat heart and mouse heart.
  • 特記事項

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 15 kDa.

ターゲット情報

  • 機能Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • 配列類似性Belongs to the complex I NDUFB6 subunit family.
  • 細胞内局在Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B17 antibody
    • CI antibody
    • CI B17 antibody
    • CI-B17 antibody
    • Complex I mitochondrial respiratory chain B17 subunit antibody
    • Complex I-B17 antibody
    • Complex IB17 antibody
    • MGC13675 antibody
    • NADH dehydrogenase (ubiquinone) 1 beta subcomplex subunit 6 antibody
    • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 antibody
    • NADH ubiquinone oxidoreductase B17 subunit antibody
    • NADH ubiquinone oxidoreductase beta subunit 6 antibody
    • NADH-ubiquinone oxidoreductase B17 subunit antibody
    • NDUB6_HUMAN antibody
    • NDUFB6 antibody
    see all

Anti-NDUFB6 antibody [21C11BC11] 画像

  • All lanes : Anti-NDUFB6 antibody [21C11BC11] (ab110244) at 0.5 µg/ml

    Lane 1 : isolated mitochondria from Human heart at 5 µg
    Lane 2 : isolated mitochondria from cow heart at 1 µg
    Lane 3 : isolated mitochondria from rat heart at 10 µg
    Lane 4 : isolated mitochondria from mouse heart at 10 µg


    Predicted band size : 15 kDa
  • Overlay histogram showing HepG2 cells stained with ab110244 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110244, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-NDUFB6 antibody [21C11BC11] (ab110244) 使用論文

This product has been referenced in:
  • Beutner G  et al. Initiation of electron transport chain activity in the embryonic heart coincides with the activation of mitochondrial complex 1 and the formation of supercomplexes. PLoS One 9:e113330 (2014). WB ; Mouse . Read more (PubMed: 25427064) »
  • Stiburek L  et al. YME1L controls the accumulation of respiratory chain subunits and is required for apoptotic resistance, cristae morphogenesis, and cell proliferation. Mol Biol Cell 23:1010-23 (2012). Read more (PubMed: 22262461) »

See all 14 Publications for this product

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Thank you for your enquiry.

I can confirm that the following antibodies are produced in our Mitosciences laboratories:


ab14713
ab110244
ab14715
ab14745
ab14705

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"