製品の概要

  • 製品名Anti-NDUFB4 antibody [17G3D9E12]
    NDUFB4 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [17G3D9E12] to NDUFB4
  • アプリケーション適用あり: IHC-P, WB, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Rat, Cow, Human
  • 免疫原

    NDUFB4 from cow

  • ポジティブ・コントロール
    • Isolated mitochondria from Human heart. HeLa cels. Cultured Human fibroblasts.
  • 特記事項

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with staining protocol.

WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 15 kDa.
ICC/IF Use a concentration of 5 - 15 µg/ml.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with staining protocol.

Flow Cyt Use a concentration of 1 µg/ml. (heat-induced antigen-retrieval improves signal).



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • 配列類似性Belongs to the complex I NDUFB4 subunit family.
  • 細胞内局在Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B15 antibody
    • CI-B15 antibody
    • Complex I B15 subunit antibody
    • Complex I-B15 antibody
    • NADH dehydrogenase (ubiquinone) 1 beta subcomplex 4 15kDa antibody
    • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 antibody
    • NADH ubiquinone oxidoreductase B15 subunit antibody
    • NADH-ubiquinone oxidoreductase B15 subunit antibody
    • NDUB4_HUMAN antibody
    • NDUFB4 antibody
    see all

Anti-NDUFB4 antibody [17G3D9E12] 画像

  • All lanes : Anti-NDUFB4 antibody [17G3D9E12] (ab110243) at 0.5 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from cow heart at 1 µg
    Lane 3 : Isolated mitochondria from rat heart at 10 µg


    Predicted band size : 15 kDa
  • Mitochondrial localization of complex I visualized by immunocytochemistry using aab110243.
    Cultured Human fibroblasts were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110243 at 15µg/ml, followed by a fluorescent goat-anti-mouse IgG.

  • HeLa cells were stained with 1 µg/mL ab110243 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • Immunohistochemistical detection of Anti-NDUFB4 antibody [17G3D9E12] (ab110243; 1/100, incubated for 1hr) on formaldehyde fixed human cerebellum sections. Antigen retrieval step: Heat mediated. Permeabilization: No. ab110243 was used to investigate respiratory deficiency in 5µm cut sections of FFPE human cerebellum. Sections were cut and mounted on to glass adhesion slides for staining. The antibody stained specifically, identifying both normal neurons as well as those exhibiting respiratory deficiency. Non-specific background staining was not evident, and positive staining was crisp and punctate. Detection - Menarini Diagnostics X-Cell Plus HRP-Polymer Kit. SigmaFast DAB tablets were used for the final stage of staining.

Anti-NDUFB4 antibody [17G3D9E12] (ab110243) 使用論文

This product has been referenced in:
  • Chang HY  et al. Combination therapy targeting ectopic ATP synthase and 26S proteasome induces ER stress in breast cancer cells. Cell Death Dis 5:e1540 (2014). IF ; Human . Read more (PubMed: 25429617) »
  • Hämäläinen RH  et al. Tissue- and cell-type-specific manifestations of heteroplasmic mtDNA 3243A>G mutation in human induced pluripotent stem cell-derived disease model. Proc Natl Acad Sci U S A 110:E3622-30 (2013). Read more (PubMed: 24003133) »

See all 11 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain - Cerebellum)
Specification Brain - Cerebellum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH8.0 EDTA
Permeabilization No
Username

Ms. Philippa Hepplewhite

Verified customer

投稿 Jun 28 2012

Thank you for your enquiry,

I am sorry to confirm that this kit is no longer available, and apologise for the inconvenience.

I can confirm that we do sell the separate antibody components which you may like to consider:

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Thank you for your enquiry. It has taken some time to confirm all the details for you, and I appreciate your patience.

I am sorry to confirm that MS602 has been discontinued and we do not have anymore in stock.

Although that produ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"