Anti-NDUFAF1 抗体 [EPR2796] (ab79826)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2796] to NDUFAF1
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-NDUFAF1 antibody [EPR2796]
NDUFAF1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2796] to NDUFAF1 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human NDUFAF1 aa 300-400 (C terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: HeLa, Jurkat and heart lysates. IHC-P: Human liver tissue. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2796 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab79826の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/250.
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WB |
1/10000 - 1/50000. Detects a band of approximately 33 kDa (predicted molecular weight: 38 kDa).
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ICC/IF |
1/100 - 1/500.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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Flow Cyt (Intra)
1/250. |
WB
1/10000 - 1/50000. Detects a band of approximately 33 kDa (predicted molecular weight: 38 kDa). |
ICC/IF
1/100 - 1/500. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the CIA30 family. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 51103 Human
- Entrez Gene: 69702 Mouse
- Entrez Gene: 296086 Rat
- Omim: 606934 Human
- SwissProt: Q9Y375 Human
- SwissProt: Q9CWX2 Mouse
- Unigene: 106529 Human
- Unigene: 5390 Mouse
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別名
- CGI 65 antibody
- CGI65 antibody
- CIA30 antibody
see all
画像
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All lanes : Anti-NDUFAF1 antibody [EPR2796] (ab79826) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Human heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP-conjugated at 1/2000 dilution
Predicted band size: 38 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling NDUFAF1 with purified 79826 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
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Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling NDUFAF1 (red) with ab79826 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
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Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human liver tissue using 1/100 ab79826.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunofluorescence analysis of HeLa cells using 1/100 ab79826.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab79826 は 3 報の論文で使用されています。
- Wongkitikamjorn W et al. Gestational Intermittent Hypoxia Induces Mitochondrial Impairment in the Geniohyoid Muscle of Offspring Rats. Cureus 14:e25088 (2022). PubMed: 35600069
- Miwa S et al. Low abundance of the matrix arm of complex I in mitochondria predicts longevity in mice. Nat Commun 5:3837 (2014). WB ; Mouse . PubMed: 24815183
- Ekman M et al. HIF-mediated metabolic switching in bladder outlet obstruction mitigates the relaxing effect of mitochondrial inhibition. Lab Invest 94:557-68 (2014). PubMed: 24589856