Anti-NDUFAF1 抗体 [EPR2796] (ab79826)
製品の概要
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製品名Anti-NDUFAF1 antibody [EPR2796]
NDUFAF1 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [EPR2796] to NDUFAF1
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由来種Rabbit
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アプリケーション適用あり: WB, IP, ICC/IF, Flow Cyt, IHC-Pmore details
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種交差性交差種: Mouse, Rat, Human
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免疫原
Synthetic peptide within Human NDUFAF1 aa 300-400 (C terminal). The exact sequence is proprietary.
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ポジティブ･コントロール
- WB: HeLa, Jurkat and heart lysates. IHC-P: Human liver tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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精製度Tissue culture supernatant
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ポリ/モノモノクローナル
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クローン名EPR2796
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アイソタイプIgG
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研究分野
関連製品
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Alternative Versions
アプリケーション
Our Abpromise guarantee covers the use of ab79826 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 33 kDa (predicted molecular weight: 38 kDa). | |
| IP | 1/40. | |
| ICC/IF | 1/100 - 1/500. | |
| Flow Cyt | 1/250. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能Chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).
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組織特異性Ubiquitous.
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配列類似性Belongs to the CIA30 family.
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細胞内局在Mitochondrion.
- Information by UniProt
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参照データベース
- Entrez Gene: 51103 Human
- Entrez Gene: 69702 Mouse
- Entrez Gene: 296086 Rat
- Omim: 606934 Human
- SwissProt: Q9Y375 Human
- SwissProt: Q9CWX2 Mouse
- Unigene: 106529 Human
- Unigene: 5390 Mouse
see all -
別名
- CGI 65 antibody
- CGI65 antibody
- CIA30 antibody
see all
画像
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![Immunocytochemistry/ Immunofluorescence - Anti-NDUFAF1 antibody [EPR2796] (ab79826)](http://a.abcam.com/ps/products/79/ab79826/Images/ab79826-262047-ab79826-Hela-Methanol-500.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NDUFAF1 antibody [EPR2796] (ab79826)Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling NDUFAF1 with purified 79826 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
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![Western blot - Anti-NDUFAF1 antibody [EPR2796] (ab79826)](http://a.abcam.com/ps/products/79/ab79826/Images/NDUFAF1-Primary-antibodies-ab79826-1.jpg)
Western blot - Anti-NDUFAF1 antibody [EPR2796] (ab79826)All lanes : Anti-NDUFAF1 antibody [EPR2796] (ab79826) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Human heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti Rabbit HRP-conjugated at 1/2000 dilution
Predicted band size: 38 kDa
Observed band size: 33 kDa (why is the actual band size different from the predicted?) -
![Flow Cytometry - Anti-NDUFAF1 antibody [EPR2796] (ab79826)](http://a.abcam.com/ps/products/79/ab79826/Images/ab79826-5-ab79826FC1.jpg)
Flow Cytometry - Anti-NDUFAF1 antibody [EPR2796] (ab79826)Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling NDUFAF1 (red) with ab79826 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAF1 antibody [EPR2796] (ab79826)](http://a.abcam.com/ps/products/79/ab79826/Images/NDUFAF1-Primary-antibodies-ab79826-2.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAF1 antibody [EPR2796] (ab79826)Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human liver tissue using 1/100 ab79826. -
![Immunocytochemistry/ Immunofluorescence - Anti-NDUFAF1 antibody [EPR2796] (ab79826)](http://a.abcam.com/ps/products/79/ab79826/Images/NDUFAF1-Primary-antibodies-ab79826-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NDUFAF1 antibody [EPR2796] (ab79826)Immunofluorescence analysis of HeLa cells using 1/100 ab79826.
プロトコール
参考文献
This product has been referenced in:
- Miwa S et al. Low abundance of the matrix arm of complex I in mitochondria predicts longevity in mice. Nat Commun 5:3837 (2014). WB ; Mouse . Read more (PubMed: 24815183) »
- Ekman M et al. HIF-mediated metabolic switching in bladder outlet obstruction mitigates the relaxing effect of mitochondrial inhibition. Lab Invest 94:557-68 (2014). Read more (PubMed: 24589856) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"