製品の概要

  • 製品名Anti-NDUFAF1 antibody [EPR2795]
    NDUFAF1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2795] to NDUFAF1
  • アプリケーション適用あり: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human NDUFAF1

  • ポジティブ・コントロール
    • HeLa, 293T, A549 and HepG2 cell lysates. Human liver tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Predicted molecular weight: 38 kDa.
IP 1/50.
IHC-P 1/100 - 1/250.
Flow Cyt 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/500 - 1/1000.

ターゲット情報

  • 機能Chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).
  • 組織特異性Ubiquitous.
  • 配列類似性Belongs to the CIA30 family.
  • 細胞内局在Mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CGI 65 antibody
    • CGI65 antibody
    • CIA30 antibody
    • CIA30_HUMAN antibody
    • Complex I intermediate associated protein 30 antibody
    • Complex I intermediate associated protein 30 mitochondrial antibody
    • Complex I intermediate-associated protein 30, mitochondrial antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex assembly factor 1 antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1 antibody
    • NADH:ubiquinone oxidoreductase complex assembly factor 1 antibody
    • NDUFAF 1 antibody
    • Ndufaf1 antibody
    see all

Anti-NDUFAF1 antibody [EPR2795] 画像

  • All lanes : Anti-NDUFAF1 antibody [EPR2795] (ab92454) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 38 kDa
  • Paraffin embedded Human liver tissue labelled with ab92454 at 1/100 dilution.
  • Immunofluorescence analysis of HeLa cells labelled with ab92454 at 1/500 dilution.

Anti-NDUFAF1 antibody [EPR2795] (ab92454) 使用論文

This product has been referenced in:
  • Lindfors C  et al. Hypothalamic mitochondrial dysfunction associated with anorexia in the anx/anx mouse. Proc Natl Acad Sci U S A 108:18108-13 (2011). WB . Read more (PubMed: 22025706) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"