製品の概要

  • 製品名Anti-NDUFA9 antibody
    NDUFA9 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to NDUFA9
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment: RVFEISPFEP WITRDKVERM HITDMKLPHL PGLEDLGIQA TPLELKAIEV LRRHRTYRWL SAEIEDVKPA KTVNI, corresponding to amino acids 303-378 of Human NDUFA9

  • ポジティブ・コントロール
    • WB: HAP1 and NIH/3T3 cells IHC: Human small Intestine tissue ICC/IF: NIH/3T3 cells FC: HepG2 cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 43 kDa.
IHC-P Use a concentration of 8 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • 配列類似性Belongs to the complex I NDUFA9 subunit family.
  • 細胞内局在Mitochondrion matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CI-39kD antibody
    • Complex I subunit NDUFA9 antibody
    • Complex I-39kD antibody
    • mitochondrial antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9 antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9 mitochondrial antibody
    • NADH Ubiquinone Oxidoreductase 1 alpha subcomplex 9 antibody
    • NADH-ubiquinone oxidoreductase 39 kDa subunit antibody
    • NDUA9_HUMAN antibody
    • NDUFA9 antibody
    see all

Anti-NDUFA9 antibody 画像



  • Predicted band size : 43 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: NDUFA9 knockout HAP1 cell lysate (20 µg)

    Lanes 1 - 2: Merged signal (red and green). Green - ab55521 observed at 40 kDa. Red - loading control, ab18251, observed at 52 kDa.

    ab55521 was shown to recognize NDUFA9 when NDUFA9 knockout samples were used, along with additional cross-reactive bands. Wild-type and NDUFA9 knockout samples were subjected to SDS-PAGE. ab55521 and ab18251 (loading control to alpha tubulin) were diluted at 1 µg/mL and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemical analysis of formalin fixed and paraffin embedded human small Intestine tissue labeling NDUFA9 with ab55521 at 8ug/mL.

  • Anti-NDUFA9 antibody (ab55521) at 1 µg + NIH/3T3 cell lysate at 25 µg

    Predicted band size : 43 kDa
  • Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 cells labeling NDUFA9 with ab55521 at 10 ug/mL.

  • Overlay histogram showing HepG2 cells stained with ab55521 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55521, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-NDUFA9 antibody (ab55521) 使用論文

This product has been referenced in:
  • Ahn BH  et al. A role for the mitochondrial deacetylase Sirt3 in regulating energy homeostasis. Proc Natl Acad Sci U S A 105:14447-52 (2008). WB ; Human . Read more (PubMed: 18794531) »

See 1 Publication for this product

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