製品の概要

  • 製品名Anti-NDUFA5 antibody
    NDUFA5 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to NDUFA5
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Human
  • 免疫原

    Fusion protein:

    AYRKYTEQITNEKLDMVKAEPDVKKLEALLQGGEVEEVILQAEKELSLAR KMLKWKPWEPLVEEPPANQWKWPI

    , corresponding to amino acids 43/116 of Mouse NDUFA5

  • 特記事項Produced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab22096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 13 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 機能Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • 組織特異性Expressed in all tissues examined with highest levels in heart, skeletal muscle and brain.
  • 配列類似性Belongs to the complex I NDUFA5 subunit family.
  • 細胞内局在Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B13 antibody
    • CI 13kB antibody
    • CI-13kD-B antibody
    • Complex I subunit B13 antibody
    • Complex I-13kD-B antibody
    • DKFZp781K1356 antibody
    • FLJ12147 antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 (13kD, B13) antibody
    • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa antibody
    • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 antibody
    • NADH-ubiquinone oxidoreductase 13 kDa-B subunit antibody
    • NDUA5_HUMAN antibody
    • NDUFA5 antibody
    • NUFM antibody
    • Type I dehydrogenase antibody
    • Ubiquinone reductase antibody
    • UQOR13 antibody
    see all

Anti-NDUFA5 antibody (ab22096) 使用論文

ab22096 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"