製品の概要

  • 製品名Anti-NDRG2 antibody
    NDRG2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NDRG2
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow, Pig, Chimpanzee, Cynomolgus Monkey, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human NDRG2.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Human Brain; Human Heart; Human Skeletal Muscle; Mouse Brain; Rat Brain.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab60097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37-45 kDa (predicted molecular weight: 41 kDa).
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能May be involved in dendritic cell and neuron differentiation. May have anti-tumor activity.
  • 組織特異性Highly expressed in brain, heart, skeletal muscle and salivary gland, and moderately in kidney and liver. Expressed in dendritic cells, but not in other blood cells. Expression levels are low in pancreatic and liver cancer tissues; absent in meningioma. Expressed in low-grade gliomas but present at low levels in glioblastoma. Isoform 1 and isoform 2 are present in brain neurons and up-regulated in Alzheimer disease (at protein level).
  • 配列類似性Belongs to the NDRG family.
  • 発生段階Specifically expressed during dendritic cell differentiation (in vitro). Expression is low in fetal brain and increases during brain postnatal development.
  • 細胞内局在Cytoplasm. Cytoplasm > perinuclear region. Perinuclear in neurons.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Antidepressant related protein ADRG123 antibody
    • Cytoplasmic protein Ndr1 antibody
    • DKFZp781G1938 antibody
    • FLJ25522 antibody
    • KIAA1248 antibody
    • N myc downstream regulated gene 2 antibody
    • N myc downstream regulator 2 antibody
    • NDR1 related protein NDR2 antibody
    • NDRG 2 antibody
    • NDRG family member 2 antibody
    • NDRG1 related protein antibody
    • NDRG2 antibody
    • NDRG2_HUMAN antibody
    • Protein NDRG2 antibody
    • Protein Syld709613 antibody
    • SYLD antibody
    • Syld709613 protein antibody
    see all

Anti-NDRG2 antibody 画像

  • All lanes : Anti-NDRG2 antibody (ab60097) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human heart tissue lysate - total protein (ab29431)
    Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 4 : Brain (Mouse) Tissue Lysate
    Lane 5 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 37-45 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • ICC/IF image of ab60097 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab60097 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-NDRG2 antibody (ab60097) 使用論文

ab60097 has not yet been referenced specifically in any publications.

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