製品の概要

  • 製品名Anti-NDRG2 antibody
    NDRG2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NDRG2
  • アプリケーション適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Xenopus laevis, Chimpanzee
  • 免疫原

    Synthetic peptide, corresponding to a region within C terminal amino acids 300-350 (GYMASSCMTR LSRSRTASLT SAASVDGNRS RSRTLSQSSE SGTLSSGPPG) of Human NDRG2 (NP_057334; UniProt Q9UN36 isoform 2).

  • ポジティブ・コントロール
    • WB: HepG2 cell lysate IHC-P: Human liver lysate This antibody gave a positive result when used in the following formaldehyde fixed cell lines: U87-MG.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab118927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能May be involved in dendritic cell and neuron differentiation. May have anti-tumor activity.
  • 組織特異性Highly expressed in brain, heart, skeletal muscle and salivary gland, and moderately in kidney and liver. Expressed in dendritic cells, but not in other blood cells. Expression levels are low in pancreatic and liver cancer tissues; absent in meningioma. Expressed in low-grade gliomas but present at low levels in glioblastoma. Isoform 1 and isoform 2 are present in brain neurons and up-regulated in Alzheimer disease (at protein level).
  • 配列類似性Belongs to the NDRG family.
  • 発生段階Specifically expressed during dendritic cell differentiation (in vitro). Expression is low in fetal brain and increases during brain postnatal development.
  • 細胞内局在Cytoplasm. Cytoplasm > perinuclear region. Perinuclear in neurons.
  • Information by UniProt
  • 参照データベース
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Anti-NDRG2 antibody 画像

  • ICC/IF image of ab118927 stained U87-MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab118927 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-NDRG2 antibody (ab118927) at 1 µg/ml

    Lane 1 : Mwt marker
    Lane 2 : HepG2 cell lysate at 10 µg


    Predicted band size : 39 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling NDRG2 with ab118927 at 5µg/ml.

Anti-NDRG2 antibody (ab118927) 使用論文

ab118927 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"