The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報WB: Use at a concentration of 1-5 µg/ml.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
機能Required for centrosome duplication. Essential for the development of the cerebral cortex. May regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex (By similarity). Required for formation and function of the mitotic spindle.
配列類似性Belongs to the nudE family.
翻訳後修飾Phosphorylated in mitosis.
細胞内局在Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cleavage furrow. Localizes to the interphase centrosome and to the mitotic spindle. Concentrates at the plus ends of microtubules coincident with kinetochores in metaphase and anaphase in a CENPF-dependent manner. Also localizes to the cleavage furrow during cytokinesis.
LIS1 interacting protein NUDE1 rat homolog antibody
LIS1 interacting protein NUDE1 antibody
NDE 1 antibody
Nuclear distribution gene E homolog 1 antibody
Nuclear distribution protein nudE homolog 1 antibody
NUDE 1 antibody
NudE nuclear distribution gene E homolog 1 (A. nidulans) antibody
NudE nuclear distribution gene E homolog 1 antibody
Anti-NDE1 antibody 画像
Western blot - NDE1 antibody (ab57430)
Predicted band size : 38 kDa NDE1 antibody (ab57430) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Anti-NDE1 antibody (ab57430) 使用論文
This product has been referenced in:
Bradshaw NJ et al. NDE1 and NDEL1: multimerisation, alternate splicing and DISC1 interaction. Neurosci Lett449:228-33 (2009).
Read more (PubMed: 19000741) »
Brophy TM et al. RN181, a novel ubiquitin E3 ligase that interacts with the KVGFFKR motif of platelet integrin alpha(IIb)beta3. Biochem Biophys Res Commun369:1088-93 (2008).
Read more (PubMed: 18331836) »