Anti-NCR3 抗体 [y4E6] (ab26250)

製品の概要

  • 製品名
    Anti-NCR3 antibody [y4E6]
    NCR3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [y4E6] to NCR3
  • 特異性
    This antibody is specific for NCR3 and does not cross react with NKp46 and NKp44
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: extracellular domain(19-138aa) of human NCR3 purified from E. coli.

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Thimerosal (merthiolate)
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    y4E6
  • ミエローマ
    P3x63-Ag8.653
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab26250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution.
WB 1/500 - 1/2000. Predicted molecular weight: 22 kDa.

ターゲット情報

  • 機能
    Cytotoxicity-activating receptor that contributes to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. Engagement of NCR3 by BAG6 also promotes dendritic cell (DC) maturation, both through killing those DCs that did not properly acquire a mature phenotype, and inducing NK cells to release TNFA and IFNG, which promotes DC maturation.
  • 組織特異性
    Selectively expressed by all resting and activated NK cells and weakly expressed in spleen.
  • 配列類似性
    Belongs to the natural cytotoxicity receptor (NCR) family.
    Contains 1 Ig-like (immunoglobulin-like) domain.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1C7 antibody
    • Activating natural killer receptor p30 antibody
    • Activating NK A1 receptor antibody
    • CD337 antibody
    • CD337 antigen antibody
    • LY117 antibody
    • Lymphocyte antigen 117 antibody
    • MALS antibody
    • Natural cytotoxicity triggering receptor 3 antibody
    • Natural killer cell p30 related protein antibody
    • Natural killer cell p30-related protein antibody
    • Ncr3 antibody
    • NCTR3_HUMAN antibody
    • NK-p30 antibody
    • NKp30 antibody
    see all

画像

  • Anti-NCR3 antibody [y4E6] (ab26250) at 1/1000 dilution + human recombinant protein NCR3 (residues 19-138) (50ng)

    Secondary
    goat anti-mouse secondary antibody conjugated to HRP

    Predicted band size : 22 kDa

プロトコール

参考文献

ab26250 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
spleen
Blocking step
ab80436 protein block as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jul 22 2016

Thank you for your enquiry. To answer your questions: 1. Unfortunately we do not sell the NKp30 receptor in solution. 2) A recombinant fragment of the extracellular domain(19-138aa) of human NCR3 was used as the immunogen for anti-NKp30 (ab26...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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