Anti-NCR1 抗体 [MM0491-8F24] (ab89877)

製品の概要

  • 製品名
    Anti-NCR1 antibody [MM0491-8F24]
    NCR1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MM0491-8F24] to NCR1
  • アプリケーション
    適用あり: WB, IHC-Fr, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to the extracellular domain of Human NCR1.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and sterile filtered through a 0.2 µm filter.
  • ポリ/モノ
    モノクローナル
  • クローン名
    MM0491-8F24
  • アイソタイプ
    IgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab89877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa.
IHC-Fr 1/20 - 1/100.
Flow Cyt 1/50 - 1/100. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
  • 組織特異性
    Selectively expressed by both resting and activated NK cells.
  • 配列類似性
    Belongs to the natural cytotoxicity receptor (NCR) family.
    Contains 2 Ig-like (immunoglobulin-like) domains.
  • 翻訳後修飾
    N-glycosylated.
    O-glycosylated.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activating NK receptor NK p46 antibody
    • CD335 antibody
    • FLJ99094 antibody
    • hNKp46 antibody
    • Ly94 antibody
    • Lymphocyte antigen 94 antibody
    • Lymphocyte antigen 94 homolog (activating NK receptor; NK p46) antibody
    • Lymphocyte antigen 94 homolog antibody
    • Natural cytotoxicity triggering receptor 1 antibody
    • Natural killer cell p46-related protein antibody
    • NCR1 antibody
    • NCT1 antibody
    • NCTR1_HUMAN antibody
    • NK cell activating receptor antibody
    • NK cell-activating receptor antibody
    • NK-p46 antibody
    • NKp46 antibody
    see all

参考文献

ab89877 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (peripheral blood mononuclear cells (PBMC))
Permeabilization
No
Gating Strategy
lymphocytes based on SSC vs FSC
Specification
peripheral blood mononuclear cells (PBMC)
Fixation
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 14 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Osteosarcoma)
Permeabilization
No
Specification
Osteosarcoma
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 27°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 25 2015

Application
Flow Cytometry
Sample
Human Cell (PBMCs)
Permeabilization
No
Gating Strategy
Live cell and PBMC population
Specification
PBMCs
Fixation
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 16 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (NK cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
NK cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

投稿 Jun 02 2015

Thank you for contacting Abcam.

For ab89877, the isotype is IgG2b.

If there is anything else I can help you with, please let me know.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録