製品の概要

  • 製品名
    Anti-NCOR2 antibody - ChIP Grade
    NCOR2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NCOR2 - ChIP Grade
  • 特異性
    Does not react with NCoR.
  • アプリケーション
    適用あり: ChIP, ICC/IF, IP, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1165-1363 of Human NCoR2

  • ポジティブ・コントロール
    • HeLa nuclear extract

アプリケーション

Our Abpromise guarantee covers the use of ab24551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP Use at an assay dependent dilution.
ICC/IF 1/300.
IP Use at an assay dependent dilution.
WB 1/1000. Detects a band of approximately 274 kDa (predicted molecular weight: 274 kDa).

ターゲット情報

  • 機能
    Transcriptional corepressor of NR4A2/NURR1 and acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state (By similarity). Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 5 have different affinities for different nuclear receptors.
  • 組織特異性
    Ubiquitous. High levels of expression are detected in lung, spleen and brain.
  • 配列類似性
    Belongs to the N-CoR nuclear receptor corepressors family.
    Contains 2 SANT domains.
  • ドメイン
    The N-terminal region contains repression functions that are divided into three independent repression domains (RD1, RD2 and RD3). The C-terminal region contains the nuclear receptor-interacting domains that are divided in two separate interaction domains (ID1 and ID2).
    The two interaction domains (ID) contain a conserved sequence referred to as the CORNR box. This motif is required and sufficient to permit binding to unligated TR and RARS. Sequences flanking the CORNR box determine nuclear hormone receptor specificity.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CTG 26 antibody
    • CTG repeat protein 26 antibody
    • CTG26 antibody
    • N CoR2 antibody
    • N-CoR2 antibody
    • NCoR 2 antibody
    • Ncor2 antibody
    • NCOR2_HUMAN antibody
    • Nuclear Receptor Co-Repressor 2 antibody
    • Nuclear receptor corepressor 2 antibody
    • retinoic-acid-receptor-associated corepressor antibody
    • Silencing Mediator for Retanoid and Thyroid Hormone Receptors antibody
    • Silencing mediator of retinoic acid and thyroid hormone receptor antibody
    • SMAP 270 antibody
    • SMAP270 antibody
    • SMRT antibody
    • SMRTE antibody
    • SMRTE tau antibody
    • T3 receptor associating factor antibody
    • T3 receptor-associating factor antibody
    • Thyroid retinoic acid receptor associated corepressor antibody
    • Thyroid- antibody
    • TNRC 14 antibody
    • TNRC14 antibody
    • TRAC 1 antibody
    • TRAC antibody
    • TRAC1 antibody
    see all

Anti-NCOR2 antibody - ChIP Grade 画像

  • Anti-NCOR2 antibody - ChIP Grade (ab24551) at 1/1000 dilution + HeLa nuclear extract

    Predicted band size : 274 kDa
    Observed band size : 270 kDa (why is the actual band size different from the predicted?)
  • ab24551 staining NCOR2 (green) in Human Hela cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25°C. Samples were incubated with primary antibody (1/300 in PBS + 0.2% fish scale gelatin) for 20 minutes at 25°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

Anti-NCOR2 antibody - ChIP Grade (ab24551) 使用論文

This product has been referenced in:
  • Viswakarma N  et al. Transcriptional regulation of mixed lineage kinase 3 by estrogen and its implication in ER-positive breast cancer pathogenesis. Oncotarget 8:33172-33184 (2017). ChIP . Read more (PubMed: 28388540) »
  • Chen L  et al. Cyclic AMP enhances progesterone action in human myometrial cells. Mol Cell Endocrinol 382:334-43 (2014). Human . Read more (PubMed: 24161591) »

See all 13 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela cell)
Specification
Hela cell
Fixative
Methanol
Permeabilization
No
Blocking step
Fish scale gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (huh-7)
Loading amount
15000 cells
Specification
huh-7
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Oct 10 2008

Thank you for your enquiry. We qualify our "ChIP grade" antibodies in three ways: Through our; 1. In our in-house ChIP testing program, 2. In a scientific publication or 3. by a customer review. On this occasion this antibody was sourced fr...

Read More

Thank you for your enquiry. We qualify our antibodies as "ChIP grade" status in three ways 1. in our in-house ChIP testing program, 2. in a scientific publication and 3. by a customer (Abreview). Rabbit polyclonal to NCoR2, ab24551 was qualifie...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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