製品の概要

  • 製品名Anti-NCOA5 antibody
    NCOA5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NCOA5
  • アプリケーション適用あり: IP, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    A region between residues 1 and 50 of human NCOA5 (NP_066018.1).

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab70831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at 2-5 µg/mg of lysate.
WB 1/1000 - 1/5000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 関連性NCOA5 is a nuclear receptor coregulator that can have both coactivator and corepressor functions. It interacts with nuclear receptors for steroids (ESR1 and ESR2) independently of the steroid binding domain (AF2) of the ESR receptors, and with the orphan nuclear receptor NR1D2. It Is also involved in the coactivation of nuclear steroid receptors (ER) as well as the corepression of MYC/c myc in response to 17 beta estradiol (E2).
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • bA465L10.6 antibody
    • CIA antibody
    • Coactivator independent of AF 2 antibody
    • KIAA1637 antibody
    • MGC28864 antibody
    • NCoA 5 antibody
    • NCOA5 antibody
    • Nuclear receptor coactivator 5 antibody
    • OTTMUSP00000001057 antibody
    • RP23-61O3.13 antibody
    see all

Anti-NCOA5 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling NCOA5 with ab70831 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-NCOA5 antibody (ab70831) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 3 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 66 kDa
    Observed band size : 66 kDa


    Exposure time : 3 minutes
  • Detection of NCOA5 by Western Blot of Immunprecipitate.
    ab70831 at 1µg/ml staining NCOA5 in HeLa whole cell lysate immunoprecipitated using ab70831 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

Anti-NCOA5 antibody (ab70831) 使用論文

ab70831 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab70831.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"