製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to NCKIPSD
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Full length human NCKIPSD protein (NP_909119.1).

  • ポジティブ・コントロール
    • Rat brain tissue lysate, HeLa cell lysate and NCKIPSD transfected 293T cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab88467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 23024796

ターゲット情報

  • 機能
    Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1). Induces microspike formation, in vivo (By similarity). In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42 (By similarity). May play an important role in the maintenance of sarcomeres and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion.
  • 組織特異性
    Highest expression in heart, brain, skeletal muscle, kidney and liver. Lower levels in placenta, lung, small intestine and leukocytes. Weak expression in colon, thymus and spleen.
  • 関連疾患
    Note=A chromosomal aberration involving NCKIPSD/AF3p21 is found in therapy-related leukemia. Translocation t(3;11)(p21;q23) with MLL.
  • 配列類似性
    Contains 1 SH3 domain.
  • 細胞内局在
    Nucleus. Colocalizes with DRF1 at membrane ruffles, and with Nck at Z-disks in mature cardiac myocytes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 54 kDa VacA-interacting protein antibody
    • 54 kDa vimentin-interacting protein antibody
    • 90 kDa SH3 protein interacting with Nck antibody
    • AF3p21 antibody
    • dia interacting protein antibody
    • Dia-interacting protein 1 antibody
    • Diaphanous protein-interacting protein antibody
    • DIP antibody
    • DIP-1 antibody
    • DIP1 antibody
    • NCK interacting protein with SH3 domain antibody
    • NCK-interacting protein with SH3 domain antibody
    • Nckipsd antibody
    • ORF1 antibody
    • SH3 adapter protein SPIN90 antibody
    • SH3 protein interacting with Nck 90 kDa antibody
    • SPIN90 antibody
    • SPN90_HUMAN antibody
    • VIP54 antibody
    • WASLBP antibody
    • WASP-interacting SH3-domain protein antibody
    • WISH antibody
    • wiskott- Aldrich syndrome protein-interacting protein antibody
    • Wiskott-Aldrich syndrome protein-interacting protein antibody
    see all

画像

  • Anti-NCKIPSD antibody (ab88467) at 1 µg/ml + rat brain tissue lysate at 50 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 79 kDa
    Observed band size : 79 kDa
  • Anti-NCKIPSD antibody (ab88467) at 1 µg/ml + HeLa cell lysate at 50 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 79 kDa
    Observed band size : 79 kDa
  • All lanes : Anti-NCKIPSD antibody (ab88467) at 1 µg/ml

    Lane 1 : NCKIPSD transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 79 kDa
    Observed band size : 79 kDa

参考文献

This product has been referenced in:
  • Wyse MM  et al. Dia-Interacting Protein (DIP) Imposes Migratory Plasticity in mDia2-Dependent Tumor Cells in Three-Dimensional Matrices. PLoS One 7:e45085 (2012). ICC/IF . Read more (PubMed: 23024796) »

See 1 Publication for this product

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.01%TritonX-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Ben Siemsen

Verified customer

投稿 Jun 22 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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