ab94184 is a 293T cell transfected lysate in which Human Nck has been transiently over-expressed using a pCMV-Nck plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view the preparation notes.
Function: Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2.
Similarity: Contains 1 SH2 domain.
Contains 3 SH3 domains.
PTM: Phosphorylated on Ser and Tyr residues.