製品の概要

  • 製品名Anti-Nck antibody [Y531]
    Nck 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y531] to Nck
  • 特異性ab32120 recognises Nck.
  • アプリケーション適用あり: WB, IHC-P, ICC, Flow Cyt, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues close to the SH2 domain of human Nck

  • ポジティブ・コントロール
    • Hela cell lysate and human breast carcinoma
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 47 kDa.
IHC-P Use at an assay dependent concentration.
ICC 1/250 - 1/500.
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.

ターゲット情報

  • 機能Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2.
  • 配列類似性Contains 1 SH2 domain.
    Contains 3 SH3 domains.
  • 翻訳後修飾Phosphorylated on Ser and Tyr residues.
  • 細胞内局在Cytoplasm. Endoplasmic reticulum. Nucleus. Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cytoplasmic protein NCK1 antibody
    • Melanoma Nck protein antibody
    • MGC12668 antibody
    • NCK 1 antibody
    • NCK adaptor protein 1 antibody
    • NCK alpha antibody
    • NCK antibody
    • NCK tyrosine kinase antibody
    • Nck-1 antibody
    • NCK1 antibody
    • NCK1_HUMAN antibody
    • NCKalpha antibody
    • Non catalytic region of tyrosine kinase antibody
    • SH2/SH3 adaptor protein NCK alpha antibody
    • SH2/SH3 adaptor protein NCK-alpha antibody
    see all

Anti-Nck antibody [Y531] 画像

  • Overlay histogram showing Jurkat cells stained with ab32120 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32120, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Anti-Nck antibody [Y531] (ab32120) at 1/10000 dilution + Hela cell lysate

    Observed band size : 47 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.
  • ab32120 (1/100), staining human breast carcinoma by immunohistochemistry, Paraffin embedded tissue.

Anti-Nck antibody [Y531] (ab32120) 使用論文

This product has been referenced in:

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (spinal cord, motor neuron)
Permeabilization No
Specification spinal cord, motor neuron
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde
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投稿 May 16 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"