製品の概要

  • 製品名Anti-Nck antibody
    Nck 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Nck
  • 特異性Slight cross-reactivity with NCK beta (47kDa). Detected unspecified proteins at 11,16 and 75kD.
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Hamster, Cow, Human
  • 免疫原

    Synthetic peptide:

    DHVGSLSEKLAAVVNN

    conjugated to KLH, corresponding to amino acids 170-185 of Rat Nck alpha.

  • ポジティブ・コントロール
    • Non stimulated A431 cell lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab14589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use at 4µg/500ug of lysate A431 RIPA.
    WB: Use at a concentration of 1 - 4 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2.
    • 配列類似性Contains 1 SH2 domain.
      Contains 3 SH3 domains.
    • 翻訳後修飾Phosphorylated on Ser and Tyr residues.
    • 細胞内局在Cytoplasm. Endoplasmic reticulum. Nucleus. Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Cytoplasmic protein NCK1 antibody
      • Melanoma Nck protein antibody
      • MGC12668 antibody
      • NCK 1 antibody
      • NCK adaptor protein 1 antibody
      • NCK alpha antibody
      • NCK antibody
      • NCK tyrosine kinase antibody
      • Nck-1 antibody
      • NCK1 antibody
      • NCK1_HUMAN antibody
      • NCKalpha antibody
      • Non catalytic region of tyrosine kinase antibody
      • SH2/SH3 adaptor protein NCK alpha antibody
      • SH2/SH3 adaptor protein NCK-alpha antibody
      see all

    Anti-Nck antibody (ab14589) 使用論文

    ab14589 has not yet been referenced specifically in any publications.

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