The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use 0.5µl.
WB: 1/1000. Predicted molecular weight: 47 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
NCK1 is one of the adaptor proteins which mediate specific protein-protein interactions in signaling processes. Adaptor proteins usually contain several domains like SH2 and SH3 which allow specific interactions with other specific proteins. NCK1 and NCK2 show high sequence identity (68%) and have three SH3 domains and a C-terminal SH2 domain. Both of them bind receptor tyrosine kinases such as PDGFR and other tyrosine phosphorylated proteins via their SH2 domains. Various molecules which interact with SH domains of Nck and regulate signaling process of actin cytoskeleton reorganization have been identified.
Ncks are thought to have important functions in the development of mesodermal structures during embryogenesis, linked to a role in cell movement and cytoskeletal reorganization. Ncks also have a function in modulating mRNA translation at the level of initiation by interacting eukayotic initiation factor 2 (eIF2). Under stressed conditions, protein synthesis is reduced by inhibiting the activity of eIF2 through phosphorylation, transiently inhibiting recycling of eIF2 into its active form.
Lane 1 : A431 Cell Lysate 200ug Lane 2 : Precipitated from A431 Cell Lysate 200ug using ab80620 at 2ug Lane 3 : Precipitated from A431 Cell Lysate 200ug using ab80620 at 5ug Lane 4 : Precipitated from PBS using ab80620 at 5ug
Western blot - Anti-Nck 1/2 antibody [AF6D7] (ab80620)
All lanes : Anti-Nck 1/2 antibody [AF6D7] (ab80620) at 1/5000 dilution
Lane 1 : NCK1 Recombinant Protein Lane 2 : NCK2 Recombinant Protein
Predicted band size: 47 kDa
Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175
Western blot - Nck 1/2 antibody [AF6D7] (ab80620)
All lanes : Anti-Nck 1/2 antibody [AF6D7] (ab80620) at 1/1000 dilution
Lane 1 : HeLa cell lysate Lane 2 : U937 cell lysate Lane 3 : A431 cell lysate Lane 4 : SK-N-MC cell lysate Lane 5 : Ramos cell lysate Lane 6 : PC12 cell lysate