製品の概要

  • 製品名
    Anti-NCF1 (phospho S328) antibody
    NCF1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NCF1 (phospho S328)
  • 特異性
    ab111855 detects endogenous levels of NCF1 only when phosphorylated at serine 328.
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from Human NCF1 around the phosphorylation site of serine 328 (R-N-SP-V-R).

  • ポジティブ・コントロール
    • Human lymph node tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab111855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
  • 関連疾患
    Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
  • 配列類似性
    Contains 1 PX (phox homology) domain.
    Contains 2 SH3 domains.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 47 kDa autosomal chronic granulomatous disease protein antibody
    • 47 kDa neutrophil oxidase factor antibody
    • NADPH oxidase organizer 2 antibody
    • NCF 47K antibody
    • NCF-1 antibody
    • NCF-47K antibody
    • Ncf1 antibody
    • NCF1_HUMAN antibody
    • Neutrophil cytosol factor 1 antibody
    • Neutrophil cytosolic factor 1 antibody
    • neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) antibody
    • Neutrophil NADPH oxidase factor 1 antibody
    • Nox organizer 2 antibody
    • Nox organizing protein 2 antibody
    • Nox-organizing protein 2 antibody
    • NOXO2 antibody
    • p47 phox antibody
    • p47-phox antibody
    • SH3 and PX domain containing protein 1A antibody
    • SH3 and PX domain-containing protein 1A antibody
    • SH3PXD1A antibody
    see all

Anti-NCF1 (phospho S328) antibody 画像

  • ab111855 at 1/50 dilution staining NCF1 in paraffin-embedded Human Lymph node tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

プロトコール

Anti-NCF1 (phospho S328) antibody (ab111855) 使用論文

This product has been referenced in:
  • Sowrirajan B  et al. Interleukin-27 Enhances the Potential of Reactive Oxygen Species Generation from Monocyte-derived Macrophages and Dendritic cells by Induction of p47(phox). Sci Rep 7:43441 (2017). Read more (PubMed: 28240310) »

See 1 Publication for this product

Product Wall

Application
Western blot
Sample
Mouse Cell lysate - whole cell (neutrophils)
Gel Running Conditions
Reduced Denaturing (8-10%)
Loading amount
250000 cells
Treatment
100 nM PMA for 2 min
Specification
neutrophils
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 May 27 2015

Thank you for contacting us.

Yes we have antibodies to detect the p47PHOX. The common alternative namesfor p47phox is NCF1, NOXO2 and SH3PXD1A.http://www.uniprot.org/uniprot/P14598

The antibodies we have are; ab74095, ab63331, ab11...

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Vielen Dank für Ihre Anfrage. Gegen die genannten NADPH-Oxidase-Komponenten haben wir folgende Antikörper bzw. Proteine in unserem Katalog: Click here (or use the following: http://www.abcam.com/index.html?datasheet=109366). Click here (or use the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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