製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to NCE2
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow, Dog
  • 免疫原

    Peptide with sequence C-

    HLRDKEDFRNKVDD

    , corresponding to C terminal amino acids 165-178 of human NCE2 (NP_542409.1).

  • ポジティブ・コントロール
    • Mouse liver lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab87640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa).
ELISA 1/32000. Peptide ELISA
IHC-P Use a concentration of 2.5 - 3.8 µg/ml.

ターゲット情報

  • 機能
    Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
  • 組織特異性
    Widely expressed (at protein level).
  • パスウェイ
    Protein modification; protein neddylation.
  • 配列類似性
    Belongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily.
  • Information by UniProt
  • 参照データベース
  • 別名
    • NCE2 antibody
    • NEDD8 carrier protein UBE2F antibody
    • NEDD8 conjugating enzyme antibody
    • NEDD8 conjugating enzyme UBE2F antibody
    • NEDD8 protein ligase UBE2F antibody
    • NEDD8-conjugating enzyme 2 antibody
    • NEDD8-conjugating enzyme UBE2F antibody
    • ube2f antibody
    • UBE2F_HUMAN antibody
    • Ubiquitin conjugating enzyme E2F antibody
    • Ubiquitin conjugating enzyme E2F (putative) antibody
    • Ubiquitin-conjugating enzyme E2 F antibody
    see all

画像

  • Anti-NCE2 antibody (ab87640) at 0.05 µg/ml + mouse liver lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 21 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour
  • In paraffin embedded Human Placenta shows membrane staining in trophoblasts-. Recommended concentration, 2.5-3.8µg/ml.

参考文献

ab87640 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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