製品の概要

  • 製品名
    Anti-NCE2 antibody
    NCE2 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to NCE2
  • 由来種
    Goat
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Rat
    交差が予測される動物種: Mouse, Cow, Dog, Human, Pig
  • 免疫原

    Synthetic peptide:

    SKLKRDDGLKGSRT-C

    , corresponding to N terminal amino acids 6-18 of Human NCE2 (NP_542409.1.)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • Rat Pancreas lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab113415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.05 - 0.2 µg/ml. Predicted molecular weight: 21 kDa.
ELISA 1/32000.

ターゲット情報

  • 機能
    Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
  • 組織特異性
    Widely expressed (at protein level).
  • パスウェイ
    Protein modification; protein neddylation.
  • 配列類似性
    Belongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • NCE2 antibody
    • NEDD8 carrier protein UBE2F antibody
    • NEDD8 conjugating enzyme antibody
    • NEDD8 conjugating enzyme UBE2F antibody
    • NEDD8 protein ligase UBE2F antibody
    • NEDD8-conjugating enzyme 2 antibody
    • NEDD8-conjugating enzyme UBE2F antibody
    • ube2f antibody
    • UBE2F_HUMAN antibody
    • Ubiquitin conjugating enzyme E2F antibody
    • Ubiquitin conjugating enzyme E2F (putative) antibody
    • Ubiquitin-conjugating enzyme E2 F antibody
    see all

画像

  • Western blot - Anti-NCE2 antibody (ab113415)
    Western blot - Anti-NCE2 antibody (ab113415)
    Anti-NCE2 antibody (ab113415) at 0.05 µg/ml + Rat Pancreas lysate at 35 µg

    Predicted band size: 21 kDa



    Primary incubation was 1 hour. Detected by chemiluminescence.

プロトコール

参考文献

ab113415 has not yet been referenced specifically in any publications.

ab113415 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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