Anti-NCAPH2 抗体 (ab83848)

Abreviews (1)Q&A (1)

製品の概要

  • 製品名
    Anti-NCAPH2 antibody
    NCAPH2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NCAPH2
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (EYLYSLVYQA LDFISGKRRA KQLSSVQEDR ANGVASSGVP QEAENEFLSL) of human NCAPH2 (NP_689512).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • HeLa cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

ターゲット情報

  • 機能
    Regulatory subunit of the condensin-2 complex, a complex that seems to provide chromosomes with an additional level of organization and rigidity and in establishing mitotic chromosome architecture. May play a role in lineage-specific role in T-cell development.
  • 配列類似性
    Belongs to the CND2 H2 (condensin-2 subunit 2) family.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus. Chromosome. Distributed along the arms of chromosomes assembled in vivo and in vitro.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • CAP H2 antibody
    • CAP H2 subunit of the condensin II complex antibody
    • CAPH2 antibody
    • Chromosome associated protein H2 antibody
    • Chromosome-associated protein H2 antibody
    • CNDH2_HUMAN antibody
    • Condensin-2 complex subunit H2 antibody
    • hCAP H2 antibody
    • hCAP-H2 antibody
    • Kleisin beta antibody
    • Kleisin-beta antibody
    • Ncaph2 antibody
    • Non SMC condensin II complex subunit H2 antibody
    • Non-SMC condensin II complex subunit H2 antibody
    see all

画像

  • Western blot - NCAPH2 antibody (ab83848)
    Western blot - NCAPH2 antibody (ab83848)
    Anti-NCAPH2 antibody (ab83848) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 68 kDa
    Observed band size: 68 kDa
    Additional bands at: 67 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab83848 has not yet been referenced specifically in any publications.

ab83848 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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2 reviews or Q&A

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Western blot abreview for Anti-NCAPH2 antibody

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Loading amount
50 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (7)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Feb 20 2013

I am interested in trying ab83848 and ab71459 for
western in mice. May you let me know if I can be enrolled in Abtrial program?

I am very pleased to hear you would like to test ab83848 and ab71459 in mouse. These codes will each give you $349 off your next order before the expiration date. To redeem this offer, please submit an Abreview foreach antibody in mouse and include thi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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