製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to NCAM2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Chinese hamster, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human NCAM2.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Human Brain; Mouse Brain; Mouse Olfactory Bulb; Rat Olfactory Bulb.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab93452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 93 kDa).

ターゲット情報

  • 機能
    May play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons.
  • 組織特異性
    Expressed most strongly in adult and fetal brain.
  • 配列類似性
    Contains 2 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cell adhesion molecule, neural, 2 antibody
    • MGC51008 antibody
    • N CAM 2 antibody
    • N CAM 21 antibody
    • N CAM2 antibody
    • N CAM21 antibody
    • N-CAM-2 antibody
    • NCAM 2 antibody
    • NCAM 21 antibody
    • NCAM-2 antibody
    • Ncam2 antibody
    • NCAM2_HUMAN antibody
    • NCAM21 antibody
    • Neural cell adhesion molecule 2 antibody
    • OCAM GPI antibody
    • R4B12 antibody
    • RB 8 neural cell adhesion molecule antibody
    • RB8 neural cell adhesion molecule antibody
    • RNCAM antibody
    see all

画像

  • All lanes : Anti-NCAM2 antibody (ab93452) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Mouse Olfactory Bulb Lysate
    Lane 4 : Rat Olfactory Bulb Lysate
    Lane 5 : Human brain tissue lysate - total protein (ab29466) with Immunizing peptide
    Lane 6 : Brain (Mouse) Tissue Lysate with Immunizing peptide
    Lane 7 : Mouse Olfactory Bulb Lysate with Immunizing peptide
    Lane 8 : Rat Olfactory Bulb Lysate with Immunizing peptide

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 93 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,60 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

プロトコール

参考文献

ab93452 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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