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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human NCAM aa 800 to the C-terminus (C terminal).
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our Abpromise guarantee covers the use of ab75813 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50000 - 1/100000. Detects a band of approximately 180 kDa (predicted molecular weight: 93 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
|IHC-P||Use at an assay dependent concentration.|
ab75813 staining NCAM in human adipose cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 10% serum for 30 minutes at room temperature. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. A Cy3®-conjugated donkey anti-rabbit IgG polyclonal (1/800) was used as the secondary antibody.