This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Mouse Forebrain; Mouse Hippocampus; Rat Brain; Rat Cerebellum; Rat Forebrain.
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pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
antigen recognized by monoclonal antibody 5.1H11 antibody
cell adhesion molecule, neural, 1 antibody
MSK 39 antibody
NCAM 1 antibody
NCAM C antibody
Neural cell adhesion molecule 1 antibody
Neural cell adhesion molecule NCAM antibody
Western blot - Anti-NCAM antibody (ab95153)
All lanes : Anti-NCAM antibody (ab95153) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate Lane 2 : Forebrain (Mouse) Tissue Lysate Lane 3 : Mouse Hippocampus Tissue Lysate Lane 4 : Brain (Rat) Tissue Lysate Lane 5 : Cerebellum Rat Tissue Lysate Lane 6 : Rat Forebrain
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
This antibody recognises isoforms NCAM140 and NCAM180, at 140 and 180 kDa respectively. NCAM contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This banding pattern has been observed in the literature PMID:21389209. Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
IHC image of NCAM staining in Mouse foetal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab95153, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.